Effect of dendritic cell vaccine against a tongue squamous cell cancer cell line (Tca8113) in vivo and in vitro
Effect of dendritic cell vaccine against a tongue squamous cell cancer cell line (Tca8113) in vivo and in vitro
复制标题
DOI：
10.1016/j.ijom.2005.11.004
复制
发表时间：
2006-06-01
影响因子：
2.4
通讯作者：
Li, S.
中科院分区：
文献类型：
--
作者：
Wang, Z.;Hu, Q.;Li, S.
树突状细胞（Dendritic cells，DCs）是一种具有激活幼稚T淋巴细胞功能的抗原提呈细胞，被认为是一种很有前途的肿瘤免疫佐剂。在这项研究中，T淋巴细胞介导的免疫的DC疫苗诱导的Tca 8113细胞在体内和体外的效果进行了评估。DC来自人外周血单核细胞，在粒细胞-巨噬细胞集落刺激因子、白细胞介素4和/或肿瘤坏死因子α的存在下培养，并用通过冻融法制备的Tca 8113细胞裂解物刺激。在体外MTT试验中使用由这些DC活化的自体T细胞来检测它们的杀肿瘤活性，并通过施用至具有植入肿瘤（Tca 8113）的裸鼠来研究它们的体内抗肿瘤效果。从单核细胞成功地产生了足够数量的DC。DC疫苗激活的T细胞对Tca 8113细胞有杀伤作用（P < 0.01），对裸鼠移植瘤的肿瘤倍增时间有明显的延迟作用（P < 0.01），对肿瘤的生长有明显的抑制作用（P < 0.05）。这些结果表明，DC从单核细胞诱导淋巴细胞介导的免疫反应，对舌鳞癌，并可用作车辆的肿瘤抗原。
Dendritic cells (DCs), as primary antigen-presenting cells with the capacity to activate naive T lymphocytes, are considered to be promising adjuvants for immunity against cancer. In this study, the effect of T lymphocyte-mediated immunity induced by a DC vaccine against Tca8113 cells in vivo and in vitro was evaluated. DCs were from human peripheral blood monocytes cultured in the presence of granulocyte-macrophage colony stimulating factor, interleukin 4 and/or tumour necrosis factor a, and stimulated with Tca8113 cell lysate prepared by the freeze-thaw method. The autologous T cells activated by these DCs were used in an in vitro MTT assay to detect their tumouricidal activity and investigated for their anti-tumour effect in vivo by administration to nude mice with implanted tumours (Tca8113). An adequate number of DCs were successfully generated from the monocytes. The T cells activated by the DC-based vaccine killed Tca8113 cells in vitro (P < 0.01), postponed tumour doubling time of the implanted tumours in nude mice (P < 0.01) and inhibited the growth of the tumours (P < 0.05). These results show that DCs from monocytes induce a lymphocyte-mediated immune response against tongue squamous carcinoma, and could be used as a vehicle for tumour antigens.