Polymeric Cyclosporine-A Nanoparticles for Ocular Application
Polymeric Cyclosporine-A Nanoparticles for Ocular Application
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DOI：
10.1166/jbn.2011.1325
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发表时间：
2011-10-01
影响因子：
2.9
通讯作者：
Yazan, Yasemin
中科院分区：
文献类型：
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作者：
Basaran, Ebru;Demirel, Muzeyyen;Yazan, Yasemin
在本研究中，环孢菌素 A (CsA) 成功掺入阳离子 Eudragit(R) RS 100 纳米颗粒 (EPN) 中，旨在眼部应用。在 6 个月的储存期内对制备的 EPN 进行物理化学表征。体外释放测试后，绵羊被用于体内研究，其中在兽医监督下将 100 μL 制剂应用于双眼（n = 6）。以预定的时间间隔收集房水和玻璃体液样本并通过酶免疫测定（EIA）进行分析。体外释放研究表明，纳米颗粒中掺入的药物可延长释放。然而，体内结果表明，带正电荷的 EPN 可以延长 CsA 在眼睛深层（玻璃体液）中的停留时间。
In the present study, cyclosporine A (CsA) was incorporated successfully into cationic Eudragit(R) RS 100 nanoparticles (EPNs) aiming ocular application. Physicochemical characterization of the EPNs prepared was performed during the storage period of 6 months. Following in vitro release tests, sheep were used in in vivo studies where 100 mu L of formulation was applied to both eyes (n = 6) under veterinarian supervision. Aqueous and vitreous humour samples were collected at predetermined time intervals and analyzed by enzyme immune assay (EIA). In vitro relase studies showed the extended release of the incorporated drug from the nanoparticles. However in vivo results demonstrated the prolonged residence time of CsA in the deeper layers (vitreous humour) of the eye with positively charged EPNs.