Calcyon transcript expression in macaque brain
Calcyon transcript expression in macaque brain
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DOI：
10.1002/cne.10993
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发表时间：
2004-01-06
影响因子：
2.5
通讯作者：
Meador-Woodruff, JH
中科院分区：
文献类型：
--
作者：
Oakman, SA;Meador-Woodruff, JH
在猕猴脑中使用原位杂交研究了编码钙蛋白（calcyon）的转录本的解剖分布，钙蛋白是一种24 kDa的膜蛋白，与D1样多巴胺受体活化偶联，以增强细胞内钙释放。钙蛋白mRNA在灵长类动物脑内广泛分布。在新皮质中，在所有区域都发现中等密度的弥散信号，在通常对应于皮质层II和III的表面等密度带中存在强度增加。在内侧前额叶和前扣带皮层的锥体细胞层中也可以看到信号强度增加。钙蛋白mRNA大量存在于皮质下边缘区，如脑桥核、隔膜、下丘脑、杏仁核和海马。在尾状核和壳核中观察到中等水平的钙蛋白转录本表达，在苍白球中的水平较低。丘脑核，包括网状核，表达低至中等水平。在黑质的背侧部观察到非常密集的表达。许多脑干区域表达这种转录本，特别是单胺能核，包括蓝斑和中缝背核。小脑在皮质和深核中都有可检测水平的表达。尽管钙蛋白被假设为D1样受体调节与其他神经递质受体系统的“串扰”的一种手段，但值得注意的是，在与D1样神经递质无关的脑区，特别是黑质和其他多巴胺合成细胞群中检测到了丰富的钙蛋白转录物。其中很大一部分可能与报道的钙蛋白与D5受体的相关性有关，或者此外，可能表明钙蛋白作为细胞内信号转导的调节剂具有更广泛的作用。(C)2003 Wiley-Liss，Inc.
The anatomical distribution of the transcript encoding calcyon, a 24 kDa membrane protein associated with coupling D1-like dopamine receptor activation to potentiated intracellular calcium release, was examined using in situ hybridization in the macaque brain. Calcyon mRNA is found to be abundantly distributed throughout the primate brain. In neocortex, moderately dense, diffuse signal is found in all areas, with increased intensity present in a superficial isodense band corresponding generally to cortical layers II and III. Increased intensity of signal is also seen in the pyramidal cell layers of medial prefrontal and anterior cingulate cortex. Calcyon mRNA is present abundantly in subcortical limbic areas such as the nucleus accumbens, septum, hypothalamus, amygdala, and hippocampus. Moderate calcyon transcript expression is seen in caudate and putamen, with lower levels in globus pallidus. Thalamic nuclei, including the reticular nucleus, express low to moderate levels. Very dense expression is noted in the substantia nigra pars compacta. Numerous brainstem regions express this transcript, notably monoaminergic nuclei including the locus coeruleus and dorsal raphe. The cerebellum has detectable levels of expression in both cortex and deep nuclei. Although calcyon is hypothesized as a means for D1-like receptors to modulate "cross-talk" with other neurotransmitter receptor systems, it is notable that abundant calcyon transcript is detected in brain regions not associated with D1-like neurotransmission, particularly the substantia nigra pars compacta and other dopamine-synthesizing cell groups. A substantial proportion of this may relate to the reported association of calcyon with the D5 receptor, or in addition, may suggest that calcyon has a wider role as a regulator of intracellular signal transduction. (C) 2003 Wiley-Liss, Inc.