A High Level of Circulating Valine Is a Biomarker for Type 2 Diabetes and Associated with the Hypoglycemic Effect of Sitagliptin
A High Level of Circulating Valine Is a Biomarker for Type 2 Diabetes and Associated with the Hypoglycemic Effect of Sitagliptin
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高水平的循环缬氨酸是 2 型糖尿病的生物标志物，并与西格列汀的降血糖作用相关
DOI：
10.1155/2019/8247019
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发表时间：
2019-11-11
影响因子：
4.6
通讯作者：
Zheng, Hongting
中科院分区：
文献类型：
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作者：
Liao, Xiaoyu;Liu, Bingyao;Zheng, Hongting
背景高水平的支链氨基酸（BCAA）和芳香族氨基酸（AAA）与高血糖症和糖尿病发病风险增加有关。本研究旨在评估2型糖尿病（T2 D）受试者和T2 D患者以及接受降糖药西他列汀（Sit）治疗的高脂饮食（HFD-）喂养小鼠的循环缬氨酸浓度，并分析缬氨酸浓度与代谢参数的相关性。方法.通过气相色谱-质谱（GC-MS）系统分析HFD喂养小鼠的代谢组学。在53例血糖水平正常（n=19）、新诊断的T2 D（n=20）、安慰剂治疗的T2 D（n=7）或Sit治疗的T2 D（n=7）受试者中，使用市售试剂盒检测血浆缬氨酸浓度。还评估了所有参与者的生化参数。结果SIT治疗显著改变了HFD喂养小鼠的氨基酸模式，特别是通过降低BCAA缬氨酸的水平。与健康对照组相比，T2 D患者血浆缬氨酸浓度显著升高（p
Background. High levels of branched-chain amino acids (BCAAs) and aromatic amino acids (AAAs) were associated with an increased risk of hyperglycemia and the onset of diabetes. This study is aimed at assessing circulating valine concentrations in subjects with type 2 diabetes (T2D) and in T2D patients and high-fat diet- (HFD-) fed mice treated with the hypoglycemic agent sitagliptin (Sit) and analyzing the association of valine concentrations with metabolic parameters. Methods. Metabolomics in HFD-fed mice were analyzed by gas chromatography-mass spectrometry (GC-MS) systems. Plasma valine concentrations were detected with a commercial kit in 53 subjects with normal glucose levels (n=19), newly diagnosed T2D (n=20), placebo-treated T2D (n=7), or Sit-treated T2D (n=7). Biochemical parameters were also assessed in all participants. Results. Sit treatment markedly changed the pattern of amino acid in HFD-fed mice, especially by reducing the level of the BCAA valine. Compared with the healthy controls, the plasma valine concentrations were significantly higher in the T2D patients (p