Nanospheres for DNA separation chips
Nanospheres for DNA separation chips
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DOI：
10.1038/nbt939
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发表时间：
2004-02
影响因子：
46.9
通讯作者：
M. Tabuchi;M. Ueda;N. Kaji;Y. Yamasaki;Y. Nagasaki;K. Yoshikawa;K. Kataoka;Y. Baba
中科院分区：
文献类型：
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作者：
M. Tabuchi;M. Ueda;N. Kaji;Y. Yamasaki;Y. Nagasaki;K. Yoshikawa;K. Kataoka;Y. Baba
我们在这里报告的技术进行分离的DNA片段的范围广泛，具有高速和高分辨率。该方法使用纳米颗粒介质，核壳型纳米球，结合微芯片电泳过程中的加压技术。在100 s内成功分析了高达15个双酶对（kbp）的DNA片段，未观察到迁移速率的任何饱和。DNA片段在介质中迁移，同时保持其特征性分子结构。为了保证纳米球溶液中有效的DNA装载和电聚焦，我们开发了双重加压技术。最佳的压力条件和填充纳米球的浓度对于实现改进的DNA分离至关重要。
We report here a technology to carry out separations of a wide range of DNA fragments with high speed and high resolution. The approach uses a nanoparticle medium, core-shell type nanospheres, in conjunction with a pressurization technique during microchip electrophoresis. DNA fragments up to 15 kilobase pairs (kbp) were successfully analyzed within 100 s without observing any saturation in migration rates. DNA fragments migrate in the medium while maintaining their characteristic molecular structure. To guarantee effective DNA loading and electrofocusing in the nanosphere solution, we developed a double pressurization technique. Optimal pressure conditions and concentrations of packed nanospheres are critical to achieve improved DNA separations.