Genetic analysis of noroviruses taken from gastroenteritis patients, river water and oysters.
Genetic analysis of noroviruses taken from gastroenteritis patients, river water and oysters.
复制标题
对取自胃肠炎患者、河水和牡蛎的诺如病毒进行基因分析。
DOI：
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发表时间：
2004
影响因子：
2.7
通讯作者：
Tatsuo Omura
中科院分区：
文献类型：
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作者：
You Ueki;Kazuo Akiyama;Toru Watanabe;Tatsuo Omura
由于牡蛎在日本是生吃的，其受到非细菌性胃肠炎病原体的污染已成为一个严重的健康问题。众所周知，牡蛎的消化憩室中往往含有诺如病毒（NV），因此诺如病毒可能与急性胃肠炎有关。然而，由于NV不能在细胞培养物中培养，并且它们具有遗传多样性，因此NV在水生环境中的行为鲜为人知。在这项研究中，NV样本取自胃肠炎患者；来自流入牡蛎养殖区的河流；以及从那条河捕获的牡蛎。分别在衣壳和RNA依赖性RNA聚合酶（RdRp）区域分析NV样本的遗传特性。在这两个地区，从患者身上采集的菌株与从河流和牡蛎样本中采集的菌株的一致性超过 96%。这证明牡蛎被胃肠炎患者排出的NV污染。
As oysters are eaten raw in Japan, their contamination with the non-bacterial agent of gastroenteritis has become a serious health problem. As it is well known that oysters tend to concentrate noroviruses (NV) in their digestive diverticula, NV may be linked with the acute gastroenteritis. However, since NV cannot be cultivated in cell cultures, and they have genetic diversity, the behaviour of NV in the aquatic environment is little known. In this study, NV samples were taken from gastroenteritis patients; from the river flowing into the oyster-farming area; and from oysters harvested from that river. Genetic identities of NV samples were analysed in capsid and RNA-dependent RNA polymerase (RdRp) regions respectively. In both regions, strains taken from patients were >96% identical with those from river and oyster samples. This proved that oysters were contaminated with NV excreted from patients with gastroenteritis.