Synergistically increased ILC2 and Th9 cells in lung tissue jointly promote the pathological process of asthma in mice.
Synergistically increased ILC2 and Th9 cells in lung tissue jointly promote the pathological process of asthma in mice.
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DOI：
10.3892/mmr.2016.5174
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发表时间：
2016-06
影响因子：
3.4
通讯作者：
Xinyu Ying;Zhaoliang Su;Qingli Bie;Pan Zhang;Huijian Yang;Yumin Wu;Y. Xu;Jing Wu;Mengying Zhang;Shengjun Wang;Huaxi Xu
中科院分区：
文献类型：
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作者：
Xinyu Ying;Zhaoliang Su;Qingli Bie;Pan Zhang;Huijian Yang;Yumin Wu;Y. Xu;Jing Wu;Mengying Zhang;Shengjun Wang;Huaxi Xu
近年来，辅助性T细胞（Th） 9被证明是哮喘肺免疫反应的关键介质，先天淋巴样细胞2 （ILC2s）被描述为一种新型的先天免疫细胞，具有增强免疫球蛋白E （IgE）产生的能力。然而，在哮喘小鼠模型的肺系统中，ILC2s和Th9细胞之间的相互作用仍有待阐明。本研究通过检测Th9和ILC2相关细胞因子受体，研究哮喘小鼠模型肺组织对Th9和ILC2s的反应状态。本研究还探讨了哮喘小鼠模型肺组织样本中细胞因子受体表达水平与血清样本中IgE水平的关系。本研究结果表明，肺组织样本中ILC2s和Th9细胞的频率显著增加，表明发生了th2型免疫应答。此外，RAR相关孤儿受体α、白细胞介素1受体样1、转录因子PU.1和白细胞介素(IL) - 9 mRNA表达水平较高。哮喘小鼠模型中IL - 5Rα、IL - 13Rα2和高亲和性IgE受体升高，组织样本中ILC2或Th9相关受体的表达水平与血清中IgE水平呈正相关。这说明在哮喘小鼠模型肺组织中ILC2s和Th9处于极化状态，可能是相互促进的，肺组织对这两种细胞的反应是通过受体表达的增加。
In recent years, T helper (Th) 9 cells have been demonstrated to be key mediators in immune responses in asthmatic lungs, and innate lymphoid cells 2 (ILC2s) have been described as a novel type of innate immunocyte with the ability to enhance immunoglobulin E (IgE) production. However, the interaction between ILC2s and Th9 cells in the pulmonary system of a mouse model of asthma remains to be elucidated. In the present study, the response state of lung tissue with regards to Th9 and ILC2s in a mouse model of asthma was investigated by detecting Th9‑ and ILC2‑associated cytokine receptors. The present study also investigated the association between the expression levels of the cytokine receptors in lung tissue samples and the IgE levels in sera samples from mouse models of asthma. Results from the present study demonstrated that the frequency of ILC2s and Th9 cells was significantly increased in the lung tissue samples, indicating that a Th2-type immune response had occurred. In addition, high mRNA expression levels of RAR‑related orphan receptor α, interleukin 1 receptor‑like 1, transcription factor PU.1 and interleukin (IL)‑9 were observed. Furthermore, IL‑5Rα, IL‑13Rα2 and high‑affinity IgE receptor were increased in mouse models of asthma, and a positive association was observed between the expression levels of ILC2‑ or Th9‑associated receptors in tissue samples and IgE levels in the sera. This indicated that ILC2s and Th9 were in a state of polarization and may promote each other in the lung tissue of mouse models of asthma, and that the lung tissue was responding to the two types of cells via increased expression of receptors.