Phylogenetic analysis of the citrus Huanglongbing (HLB) bacterium based on the sequences of 16S rDNA and 16S/23S rDNA intergenic regions among isolates in China
Phylogenetic analysis of the citrus Huanglongbing (HLB) bacterium based on the sequences of 16S rDNA and 16S/23S rDNA intergenic regions among isolates in China
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基于中国分离株 16S rDNA 和 16S/23S rDNA 基因间区域序列的柑橘黄龙病 (HLB) 细菌系统发育分析
DOI：
10.1007/s10658-009-9436-0
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发表时间：
2009-07-01
影响因子：
1.8
通讯作者：
Yi, Ganjun
中科院分区：
文献类型：
--
作者：
Ding, Fang;Deng, Xiuxin;Yi, Ganjun
利用16 S rDNA和16 S/23 S rDNA基因间隔区序列对柑橘黄龙病（HLB）中国分离物进行了系统发育分析。对采自中国7个省、自治区、直辖市的9份不同症状的HLB样品进行PCR扩增和16 SrDNA序列测定。中国分离物的同源性为98.5%~ 100%，与印度HLB分离物Poona（GenBank登录号：L22532）的同源性相同。与非洲黄龙杆菌菌株“内尔斯普鲁伊特”（L22533）的同源性为97.5%~ 97.8%，与Ca. L.非洲亚种‘Capensis’（AF 137368）与Ca.利比里亚种'LSg 2'（AY 919312）的分离率为94.9%~ 96.0%。美洲乳杆菌来自巴西（圣保罗州; AY 742824）。利用16 S rDNA序列构建的系统发育树表明，中国分离物均属于Ca. L.亚洲人。对7个省18个不同症状的柑橘类HL B病样品进行了16 S/23 SrDNA基因间区分析。这些分离物之间没有明显的变异，同源性均大于99.0%。16 S/23 S rDNA基因间隔区序列分析和系统发育树分析表明，中国分离物与Ca. L. asiaticus，与Ca. L.africanusandCa. L.americanus.这些结果表明，中国HLB分离物属于种黄龙杆菌亚洲。这是国内首次基于分子生物学研究对HLB菌株进行分类的报道。
Phylogenetic analysis of Chinese isolates of the citrus Huanglongbing (HLB) bacterium based on the 16S rDNA and 16S/23S rDNA intergenic regions sequences was carried out. Nine HLB samples collected from different hosts with different symptoms in seven Chinese provinces, were subjected to PCR for amplifying and sequencing the 16S rDNA. The identity level among Chinese isolates was 98.5% to 100% and was the same with the Indian HLB isolate ‘Poona’ (GenBank accession number: L22532). By contrast, identity values were 97.5% to 97.8% withCandidatus Liberibacter africanusstrain ‘Nelspruit’ (L22533), 96.3% to 97.3% withCa. L. africanussubsp. ‘Capensis’ (AF137368), 95.3% to 96.5% with theCa. Liberibactersp. ‘LSg2’ (AY919312), and 94.9% to 96.0% with a strain ofCa. L.americanusfrom Brazil (São Paulo State; AY742824). A phylogenetic tree constructed with 16S rDNA sequences showed that all Chinese isolates belong toCa. L. asiaticum. Analysis of the 16S/23S rDNA intergenic region was conducted on 18 HLB-diseased citrus samples with different symptoms, collected in seven provinces. These isolates showed no obvious variation and had an identity level >99.0% with one another. Sequence analysis of 16S/23S rDNA intergenic region and the relative phylogenetic tree showed that the Chinese isolates are very close toCa. L. asiaticus, and distinct fromCa. L.africanusandCa. L.americanus. These results suggest that the Chinese HLB isolates belong to the speciesCandidatus Liberibacter asiaticus. This is the first report on the classification of HLB isolates from China based on molecular investigations.