Effect of postovulatory oocyte aging on DNA methylation imprinting acquisition in offspring oocytes
Effect of postovulatory oocyte aging on DNA methylation imprinting acquisition in offspring oocytes
复制标题
排卵后卵母细胞衰老对子代卵母细胞DNA甲基化印记获得的影响
DOI：
10.1016/j.fertnstert.2011.09.022
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发表时间：
2011-12-01
影响因子：
6.7
通讯作者：
Sun, Qing-Yuan
中科院分区：
文献类型：
--
作者：
Liang, Xing-Wei;Ge, Zhao-Jia;Sun, Qing-Yuan
目的：为了研究母亲卵母细胞的排卵后老化是否影响后代卵母细胞中印记基因的DNA甲基化获取。设计：随机研究实验研究。设置：学术基础研究实验室。动物：小鼠。干预：对来自母亲的新鲜卵母细胞和老化卵母细胞进行人工授精，并从所产生的后代中收集卵母细胞。主要结果测量：对母系印记基因Peg 3、Snrpn、Peg 1和父系印记基因H19的卵母细胞差异甲基化区域（DMR）的甲基化状态进行了评价。我们的研究结果表明，在来自老化卵母细胞后代的卵母细胞中，Peg 3、Snrpn、Peg 1和H19的DMR甲基化模式主要是正常的，结论：排卵后卵母细胞老化可导致生育能力下降，但不会导致子代卵母细胞DNA甲基化印迹的获得缺陷。（Fertil Steril（R）2011;96：1479-84. (C)美国生殖医学会（American Society for Reproductive Medicine）
Objective: To investigate whether postovulatory aging of oocytes in the mother affects DNA methylation acquisition of imprinted genes in oocytes from the offspring.Design: Randomized research experimental study.Setting: Academic basic research laboratory.Animal(s): Mice.Intervention(s): Fresh oocytes and aged oocytes from mothers were artificially inseminated, and oocytes were collected from the resultant offspring.Main Outcome Measure(s): Methylation status was evaluated at differentially methylated regions (DMRs) in oocytes of maternally imprinted genes Peg3, Snrpn, and Peg1 and paternally imprinted gene H19.Result(s): Our results showed that methylation patterns at DMRs of Peg3, Snrpn, Peg1, and H19 in oocytes from aged-oocyte offspring were mainly normal, with only a small number of oocytes showing aberrant methylation in the DMR of Peg3.Conclusion(s): Postovulatory oocyte aging causes a decline in reproductive outcomes but does not evidently lead to defects in DNA methylation imprinting acquisition in the oocytes from viable offspring. (Fertil Steril (R) 2011;96:1479-84. (C) 2011 by American Society for Reproductive Medicine.)