Elevated circulating platelet-derived microparticles in patients with active inflammatory bowel disease
Elevated circulating platelet-derived microparticles in patients with active inflammatory bowel disease
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DOI：
10.1111/j.1572-0241.2005.50381.x
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发表时间：
2005-09-01
影响因子：
9.8
通讯作者：
Fujiyama, Y
中科院分区：
文献类型：
--
作者：
Andoh, A;Tsujikawa, T;Fujiyama, Y
目的：血小板衍生微粒(PDMPs)是参与止血和炎症反应的活性分子。为了评价炎症性肠病(IBD)患者血小板功能的变化，我们测定了循环PDMP水平。方法：对25例健康对照、44例溃疡性结肠炎(UC)和43例克罗恩病(CD)患者进行研究。采用双抗体夹心酶联免疫吸附试验(ELISA)检测血浆PDMP和可溶性P-选择素(sP-选择素)水平。结果：健康对照组血浆PDMP水平为17.2+/-6.2U/mL。健康对照组与非活动期UC患者(20.8+/-9.5U/m L，n=25)和非活动期CD患者(17.6+/-7.8U/m L，n=24)比较，差异均无统计学意义。活动期UC(49.2+/-33.6U/m L，n=19)和活动期CD(48.6+/-42.8U/m L，n=19)患者的PDMP水平均显著高于健康对照组。UC患者PDMP水平与临床活动指数(CAI)呈正相关(r=0.65，p<；0.01，n=44)，与克罗恩病活动指数(CDAI)呈显著正相关(r=0.72，p<；0.01，n=43)。活动期患者缓解后升高的PDMP水平明显降低。PDMP水平与sP-选择素水平呈显著正相关(r=0.60，p<；0.01，n=122)。结论：活动期IBD患者外周血中PDMP水平升高，提示血小板在IBD发病机制中起一定作用。
OBJECTIVES: Platelet-derived microparticles (PDMPs) are active molecules involved in the hemostatic and inflammatory responses. To evaluate the changes in the platelet function in patients with inflammatory bowel disease (IBD), we measured circulating PDMP levels.METHODS: Twenty-five healthy controls, 44 patients with ulcerative colitis (UC), and 43 patients with Crohn's Disease (CD) were studied. The PDMP and soluble P-selectin (sP-selectin) levels were measured by enzyme-linked immunosorbent assay (ELISA).RESULTS: In the healthy controls, the PDMP levels were 17.2 +/- 6.2 U/mL. Significant differences were not observed between the healthy controls and inactive UC patients (20.8 +/- 9.5 U/mL, n = 25) or between the healthy controls and inactive CD patients (17.6 +/- 7.8 U/mL, n = 24). In contrast, the PDMP levels were significantly higher in both active UC (49.2 +/- 33.6 U/mL, n = 19) and active CD (48.6 +/- 42.8 U/mL, n = 19) patients than in the healthy controls. A significant correlation was found between the PDMP levels and the clinical activity indexes (CAI) of UC patients (r = 0.65, p < 0.01, n = 44), and between the PDMP levels and Crohn's disease activity indexes (CDAI) (r = 0.72, p < 0.01, n = 43). Elevated PDMP levels in active patients were significantly reduced after remission. A significant correlation was observed between the PDMP levels and the sP-selectin levels (r = 0.60, p < 0.01, n = 122).CONCLUSION: Elevated circulating PDMPs in active IBD patients suggest a role for platelets in the pathogenesis of IBD.