Global Post-Translational Modification Discovery.
Global Post-Translational Modification Discovery.
复制标题
全球翻译后修饰发现。
DOI：
10.1021/acs.jproteome.6b00034
复制
发表时间：
2017-04-07
影响因子：
4.4
通讯作者：
Smith LM
中科院分区：
文献类型：
--
作者：
Li Q;Shortreed MR;Wenger CD;Frey BL;Schaffer LV;Scalf M;Smith LM
描述了一种新的全局翻译后修饰（PTM）发现策略G-PTM-D。包含UniProt-curated PTM信息的蛋白质组学数据库补充了从具有超宽前体质量耐受性的质谱数据的第一轮搜索中发现的潜在新修饰类型和位点。采用标准窄质量公差进行的补充数据库进行的第二轮搜索在复杂的未富集样品中产生深度覆盖和具有高置信度的丰富多样的肽修饰。G-PTM-D策略代表了先前报道的G-PTM策略的重大进展，并为蛋白质组学研究界提供了强大的新能力。
A new global post-translational modification (PTM) discovery strategy, G-PTM-D, is described. A proteomics database containing UniProt-curated PTM information is supplemented with potential new modification types and sites discovered from a first-round search of mass spectrometry data with ultrawide precursor mass tolerance. A second-round search employing the supplemented database conducted with standard narrow mass tolerances yields deep coverage and a rich variety of peptide modifications with high confidence in complex unenriched samples. The G-PTM-D strategy represents a major advance to the previously reported G-PTM strategy and provides a powerful new capability to the proteomics research community.