Correlation between miR-103 and miR-133a Expression and the Circulating ANGPTL8 in Type 2 Diabetic Patients and Healthy Control Subjects
Correlation between miR-103 and miR-133a Expression and the Circulating ANGPTL8 in Type 2 Diabetic Patients and Healthy Control Subjects
复制标题
DOI：
10.7754/clin.lab.2019.190436
复制
发表时间：
2019-01-01
影响因子：
0.7
通讯作者：
Tavilani, Heidar
中科院分区：
文献类型：
--
作者：
Ghasemi, Hassan;Karimi, Jamshid;Tavilani, Heidar
背景资料：血管生成素样蛋白8（Angiopoietin-like protein 8，ANGPTL 8）是一种循环激素，在胰腺β细胞的增殖和脂质代谢中起重要作用。microRNAs（miRs）是一类在糖尿病发病机制中起重要作用的非编码小分子RNA。因此，我们研究了miR-103和miR-133 a表达与ANGPTL 8和其他2型糖尿病（T2 DM）相关因素的相关性。结果：T2 D患者外周血ANGPTL 8浓度和miR-103/133 a表达均显著高于健康对照组（p < 0.05）。在T2 D组中，miR-103/133 a与甘油三酯（TG）、总胆固醇、空腹血糖（FBS）和糖化血红蛋白之间存在正相关和显著相关（p < 0.05）。结论：miR-103/133 a的表达与ANGPTL 8及T2 D相关因素有关。
Background: Angiopoietin-like protein 8 (ANGPTL8) is a circulatory hormone that plays an important role in the proliferation of the pancreatic beta cells and lipid metabolism. MicroRNAs (miRs) are small non-coding RNAs that play an important role in the pathogenesis of diabetes mellitus. Therefore, we investigated the correlation of miR-103 and miR-133a expression with the ANGPTL8 and other type 2 diabetes mellitus (T2DM)-related factors.Methods: Seventy subjects (controls: n = 35 and type 2 diabetic patients: n = 35) participated in this study. The ANGPTL8 concentration and miR-103/133a expression were measured using ELISA and real-time PCR, respectively.Results: The circulatory ANGPTL8 concentration and miR-103/133a expression was significantly higher in T2D patients than in healthy controls (p < 0.05). There was a positive and significant correlation between miR-103/133a with triglycerides (TG), total cholesterol, fasting blood sugar (FBS), and glycated hemoglobin (p < 0.05) in the T2D group. The results also showed a negative and significant correlation between miR-103/133a expression with ANGPTL8 levels in the T2D group (p < 0.05).Conclusions: Our results suggest that miR-103/133a expression is correlated with the ANGPTL8 and T2D-related factors.