Expression and functional significance of adhesion molecules on cultured endothelial cells in response to ionizing radiation.
Expression and functional significance of adhesion molecules on cultured endothelial cells in response to ionizing radiation.
复制标题
培养的内皮细胞响应电离辐射的粘附分子的表达和功能意义。
DOI：
10.1038/sj/mn/7800105
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Kiani,MF
中科院分区：
文献类型：
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作者：
Prabhakarpandian,B;Goetz,DJ;Swerlick,RA;Chen,X;Kiani,MF
已显示辐射后内皮细胞上粘附分子的上调，但这种上调在各种内皮细胞系中的功能意义尚不清楚。我们已经开发了一个体外流动模型，研究辐射诱导的上调E-选择素和细胞间粘附分子（ICAM-1）的功能后果。MethodsHuman真皮微血管内皮细胞（HDMEC），人脐静脉内皮细胞（HUVEC），或转化的人微血管内皮细胞（HMEC-1）生长在35毫米的培养皿和照射单剂量为10戈伊。HL-60（人早幼粒细胞白血病）细胞在平行板流动室中在范围从0.5至2.0达因/cm 2的剪切应力下灌注在照射的内皮细胞上。流式细胞术被用来量化的表达E-选择素和ICAM-1上的各种内皮cells.ResultsFlow cytomeric分析显示上调ICAM-1的表达对所有三种细胞类型postirradiation（后IR），和一个上调的E-选择素的表达只对HDMEC后IR。E-选择素的表达检测到控制HDMEC，但在一个较低的水平比检测后IR HDMEC。流式细胞术显示，在剪切力≤1.5 dynes/cm 2时，IR后HDMEC上滚动和牢固粘附的HL-60细胞数量显著增加;用E-选择素和ICAM-1抗体预处理对照和照射后的HDMEC，分别显著减少滚动和牢固粘附的HL-60细胞数量。没有滚动或坚定的粘附HL-60细胞上观察到HUVEC或HMEC-1单层post-IR.ConclusionThese研究结果表明，ICAM-1上调照射HDMEC，HUVEC，和HMEC-1。E-选择素仅在辐照的HDMEC上上调至功能水平，而在辐照的HUVEC或HMEC-1上不上调。Microcirculation（2001）8，355-364。
ObjectiveUpregulation of adhesion molecules on endothelial cells following irradiation has been shown, but the functional significance of this upregulation in various endothelial cell lines is not clear. We have developed anin vitroflow model to study the functional consequences of the radiation-induced upregulation of E-selectin and intercellular adhesion molecule (ICAM-1).MethodsHuman dermal microvascular endothelial cells (HDMEC), human umbilical vein endothelial cells (HUVEC), or transformed human microvascular endothelial cells (HMEC-1) were grown in 35-mm dishes and irradiated with a single dose of 10 Gy. HL-60 (human promyelocytic leukemia) cells were perfused over the irradiated endothelial cells in a parallel plate flow chamber at shear stress ranging from 0.5 to 2.0 dynes/cm2. Flow cytometry was used to quantify the expression of E-selectin and ICAM-1 on the various endothelial cells.ResultsFlow cytomeric analysis revealed an upregulation of ICAM-1 expression on all three cell types postirradiation (post-IR), and an upregulation of E-selectin expression only on HDMEC post-IR. E-selectin expression was detected on control HDMEC, but at a lower level than that detected on post-IR HDMEC. Flow assays revealed a significant increase in the number of rolling and firmly adherent HL-60 cells on post-IR HDMEC at shear stress ≤1.5 dynes/cm2; pretreatment of control and irradiated HDMEC with antibodies to E-selectin and ICAM-1 significantly diminished the number of rolling and firmly adherent HL-60 cells, respectively. No rolling or firm adhesion of HL-60 cells was observed on HUVEC or HMEC-1 monolayers post-IR.ConclusionThese findings suggest that ICAM-1 is upregulated on irradiated HDMEC, HUVEC, and HMEC-1. E-selectin is upregulated to a functional level only on irradiated HDMEC, and not on irradiated HUVEC or HMEC-1.Microcirculation (2001) 8, 355–364.