Bioaccumulation of silver in ectomycorrhizal and saprobic macrofungi from pristine and polluted areas
Bioaccumulation of silver in ectomycorrhizal and saprobic macrofungi from pristine and polluted areas
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DOI：
10.1016/j.scitotenv.2010.02.031
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发表时间：
2010-06-01
影响因子：
9.8
通讯作者：
Dunn, Colin E.
中科院分区：
文献类型：
--
作者：
Borovicka, Jan;Kotrba, Pavel;Dunn, Colin E.
大型真菌是银的有效蓄积者，本研究从原始数据和银污染地区大型真菌的银浓度的原始数据对这一现象进行了全面的综述。在原始地区，外生菌根(ECM)和腐生(SAP)大型真菌的中位数Ag浓度分别为079和2.94 mg kg(-1)。在ECM和SAP大型真菌中，银的超积累阈值分别为100和300 mg kg(-1)。分别进行了分析。在银污染地区，大型真菌(ECM和SAP)中的Ag含量显著升高，中位数为24.7 mg kg(-1)，阴道组鹅膏中的Ag含量最高(304~692 mg kg-1)。用体积排阻色谱、反相高效液相色谱和改进的聚丙烯酰胺凝胶电泳法分析了膜下鹅膏菌子实体内银的形态，并用反相高效液相色谱法和改进的聚丙烯酰胺凝胶电泳法进行了配体的巯基荧光分析。在分离的银复合体中缺乏谷胱甘肽和植物螯合素，并存在单一的3 kDa含硫基肽，这表明膜下隐翅虫对银的解毒作用可能依赖于金属硫蛋白。在受污染的森林土壤剖面中，银的垂直分布在有机层中表现出显著的富集性：在受污染的土壤中，银的最高浓度出现在表层。标准EDTA提取法在两种土壤剖面中均显示出相对较低的银萃取率，一般在总银含量的2.2-7.7%范围内。(C)2010爱思唯尔B.V.保留所有权利。
Macrofungi are effective accumulators of Ag This study provides a comprehensive review of this phenomenon supported by original data on the Ag concentrations of macrofungi from pristine and Ag-polluted areas. In pristine areas, the median Ag concentrations of ectomycorrhizal (ECM) and saprobic (SAP) macrofungi were 079 and 2.94 mg kg(-1), respectively In these areas. hyperaccumulation thresholds for Ag in ECM and SAP macrofungi are proposed as 100 and 300 mg kg(-1). respectively. In a Ag-polluted area, the Ag concentrations in macrofungi (ECM and SAP) were significantly elevated with the median value of 24.7 mg kg(-1) and the highest concentrations in Amanita spp of the section Vaginatae (304-692 mg kg(-1)). The intracellular speciation of Ag in fruit-bodies of the Ag-accumulator Amanita submembranacea was inspected by size exclusion chromatography followed by sulfhydryl-specific fluorimetnc assays of ligands using reverse phase high-performance liquid chromatography and improved polyacrylamide gel electrophoresis Virtually all Ag was found to be intracellular and sequestered in the major 7 kDa and minor 33 kDa complexes. The lack of glutathione and phytochelatins and the presence of a single 3 kDa sulfhydryl-containing peptide in the isolated Ag-complexes suggest that detoxification of Ag in A. submembranacea may rely on metallothionein. Vertical distribution of Ag in a polluted forest soil profile has shown substantial enrichment in organic horizons: in polluted technosol, the highest Ag concentrations were found in surface layers. Standardized EDTA extraction of Ag in both the investigated soil profiles showed relatively low Ag extractibility, generally within the range of 2 2-7 7% of total Ag content. (C) 2010 Elsevier B.V. All rights reserved.