PORPHYRIN-INDUCED ENHANCEMENT OF ULTRASOUND CYTOTOXICITY
PORPHYRIN-INDUCED ENHANCEMENT OF ULTRASOUND CYTOTOXICITY
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DOI：
10.1080/09553009414551131
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发表时间：
1994-08-01
影响因子：
2.6
通讯作者：
CAIN, C
中科院分区：
文献类型：
--
作者：
KESSEL, D;JEFFERS, R;CAIN, C
我们研究了超声波对培养的小鼠白血病 L1210 细胞系的影响，其中卟啉的存在使细胞对光敏感的能力差异很大。这些卟啉仅当存在于培养介质中时才会在体外增强超声诱导的细胞损伤。当含有细胞内卟啉的细胞暴露于超声波时，细胞活力没有改变。此外，给定卟啉对光诱导的细胞毒性与超声诱导的细胞毒性的功效之间不存在相关性。活力的丧失与氨基酸转运和细胞破碎的抑制有关，表明细胞膜完整性的破坏。超声诱导的细胞损伤仅发生在空化核存在的情况下。
We examined the effect of ultrasound on the murine leukaemia L1210 cell line in culture, in the presence of porphyrins varying widely in their capacity to sensitize cells to light. These porphyrins enhanced ultrasound-induced cell damage in vitro only when present in the incubation medium. When cells containing intracellular porphyrins were exposed to ultrasound, cell viability was not altered. Moreover, there was no correlation between the efficacy of a given porphyrin for light- versus ultrasound-induced cytotoxicity. Loss of viability was associated with inhibition of amino acid transport and cell fragmentation, suggesting disruption of the integrity of the cell membrane. Ultrasound-induced cell damage occurred only in the presence of cavitation nuclei.