Effect of dietary carbohydrates on glucagon and insulin receptors in genetically obese female Zucker rats.
Effect of dietary carbohydrates on glucagon and insulin receptors in genetically obese female Zucker rats.
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膳食碳水化合物对遗传性肥胖雌性 Zucker 大鼠胰高血糖素和胰岛素受体的影响。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
L. Recant
中科院分区：
文献类型：
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作者：
S. Bhathena;P. Aparicio;K. Revett;N. Voyles;L. Recant
肥胖Zucker大鼠是高血糖和轻度高血糖。由于胰岛素和胰高血糖素参与脂质和碳水化合物代谢，并且它们通过其受体起作用，因此我们研究了胰岛素和胰高血糖素受体在肥胖和瘦雌性Zucker大鼠中的作用。由于膳食蔗糖比淀粉更容易产生脂肪，我们还研究了膳食碳水化合物对受体的影响。观察到对血糖、甘油三酯和胰岛素血浆水平的显著表型效应（肥胖大于精瘦）。肥胖大鼠的胰岛素和胰高血糖素与肝细胞膜的结合显著低于瘦大鼠。较低的胰岛素结合是由于较低数量的受体以及较低的亲和力，而较低的胰高血糖素结合仅是由于较低的受体数量。瘦大鼠的胰岛素结合率在蔗糖喂养的大鼠中低于淀粉喂养的大鼠，而在肥胖大鼠中则不然。饮食对胰高血糖素结合无影响。我们认为，在肥胖Zucker大鼠中，除了高胰岛素血症外，胰高血糖素活性受损（表现为胰高血糖素与靶组织结合减少）可能是导致高血糖症和肥胖的重要因素。
Obese Zucker rats are hyperlipemic and mildly hyperglycemic. Because insulin and glucagon are involved in lipid and carbohydrate metabolism and they act via their receptors, we investigated the role of insulin and glucagon receptors in obese and lean female Zucker rats. Because dietary sucrose is more lipogenic than starch, we also studied the effect of dietary carbohydrates on the receptors. Significant phenotypic effect (obese greater than lean) was observed on plasma levels of glucose, triglyceride and insulin. Binding of insulin and glucagon to liver plasma membranes was significantly lower in obese rats than in lean rats. Lower insulin binding was due to a lower number of receptors as well as a lower affinity, whereas the lower glucagon binding was due only to a lower receptor number. Insulin binding in lean rats but not in obese rats was lower in sucrose-fed than in starch-fed rats. Diet had no effect on glucagon binding. We propose that in obese Zucker rats, in addition to hyperinsulinemia, impaired glucagon activity as manifested by decreased glucagon binding to target tissues may be an important contributor to the hyperlipemia and obesity.