Potent MERS-CoV Fusion Inhibitory Peptides Identified from HR2 Domain in Spike Protein of Bat Coronavirus HKU4
Potent MERS-CoV Fusion Inhibitory Peptides Identified from HR2 Domain in Spike Protein of Bat Coronavirus HKU4
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DOI：
10.3390/v11010056
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发表时间：
2019-01
期刊：
影响因子：
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通讯作者：
Shuai Xia;Qiaoshuai Lan;J. Pu;Cong Wang;Zezhong Liu;W. Xu;Qian Wang;Huan Liu;Shibo Jiang-
中科院分区：
文献类型：
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作者：
Shuai Xia;Qiaoshuai Lan;J. Pu;Cong Wang;Zezhong Liu;W. Xu;Qian Wang;Huan Liu;Shibo Jiang-
中东呼吸综合征冠状病毒（MERS-CoV）于2012年出现，并在世界范围内持续暴发，死亡率很高。然而，目前还没有有效的抗mers - cov药物。最近，大量的进化研究表明，MERS-CoV起源于蝙蝠冠状病毒（BatCoV）。我们报道了从BatCoV HKU4刺突蛋白HR2区域衍生的三个肽，包括HKU4- hr2p1， HKU4- hr2p2和HKU4- hr2p3，可以与MERS-CoV hr1衍生的肽结合形成高稳定性的六螺旋束（6-HB）。此外，这些肽，特别是HKU4- hr2p2和HKU4- hr2p3，对MERS-CoV s介导的细胞融合和病毒感染表现出强大的抑制活性，这表明这些HKU4 hr2衍生肽可能是进一步开发MERS-CoV感染抗病毒药物的候选药物。
The Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in 2012 and caused continual outbreaks worldwide with high mortality. However, no effective anti-MERS-CoV drug is currently available. Recently, numerous evolutionary studies have suggested that MERS-CoV originated from bat coronavirus (BatCoV). We herein reported that three peptides derived from the HR2 region in spike protein of BatCoV HKU4, including HKU4-HR2P1, HKU4-HR2P2 and HKU4-HR2P3, could bind the MERS-CoV HR1-derived peptide to form a six-helix bundle (6-HB) with high stability. Moreover, these peptides, particularly HKU4-HR2P2 and HKU4-HR2P3, exhibited potent inhibitory activity against MERS-CoV S-mediated cell–cell fusion and viral infection, suggesting that these HKU4 HR2-derived peptides could be candidates for futher development as antiviral agents against MERS-CoV infection.