Pax6 is essential for establishing ventral-dorsal cell boundaries in pituitary gland development
Pax6 is essential for establishing ventral-dorsal cell boundaries in pituitary gland development
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DOI：
10.1073/pnas.96.25.14378
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发表时间：
1999-12-07
影响因子：
11.1
通讯作者：
Rosenfeld, MG
中科院分区：
文献类型：
--
作者：
Kioussi, C;O'Connell, S;Rosenfeld, MG
Pax 6是一个高度保守的同源异型结构域转录因子家族成员，在眼、神经和胰腺发育中起重要作用，并在垂体发育过程中影响不对称的短暂背侧表达，其表达在特定细胞类型的腹侧->背侧出现之前消失。在小眼睛和Pax 6-/-小鼠突变体的垂体发育的分析表明，垂体的背腹轴变得腹侧化，背侧延伸的腹侧细胞类型的转录决定簇，特别是PFrk。该腹侧化之后是终末分化的背侧生长激素细胞和催乳细胞类型的显著减少以及腹侧促甲状腺细胞和促性腺激素谱系的SF-1表达细胞的标志物的表达的显著增加。我们认为，短暂的背侧表达的Pax 6是必不可少的建立一个尖锐的边界之间的背侧和腹侧细胞类型，基于抑制Shh腹侧信号。
Pax6, a highly conserved member of the paired homeodomain transcription factor family that plays essential roles in ocular, neural, and pancreatic development and effects asymmetric transient dorsal expression during pituitary development, with its expression extinguished before the ventral --> dorsal appearance of specific cell types. Analysis of pituitary development in the Small eye and Pax6 -/- mouse mutants reveals that the dorsoventral axis of the pituitary gland becomes ventralized, with dorsal extension of the transcriptional determinants of ventral cell types, particularly PFrk. This ventralization is followed by a marked decrease in terminally differentiated dorsal somatotrope and lactotrope cell types and a marked increase in the expression of markers of the ventral thyrotrope cells and SF-l-expressing cells of gonadotrope lineage. We suggest that the transient dorsal expression of Pax6 is essential for establishing a sharp boundary between dorsal and ventral cell types, based on the inhibition of Shh ventral signals.