Oxygen governs gonococcal microcolony stability by enhancing the interaction force between type IV pili
Oxygen governs gonococcal microcolony stability by enhancing the interaction force between type IV pili
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DOI：
10.1039/c5ib00018a
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发表时间：
2015-01-01
影响因子：
2.5
通讯作者：
Maier, Berenike
中科院分区：
文献类型：
--
作者：
Dewenter, Lena;Volkmann, Thorsten E.;Maier, Berenike
小细菌簇的形成，称为微菌落，是形成细菌生物膜的第一步。人类淋病奈瑟菌需要IV型菌毛（T4P）来形成微菌落并进行表面运动。在这里，我们研究了氧气对微菌落形成动力学的影响。我们发现，微菌落的形成和维持需要超过3 μ M的氧浓度。质子原动力耗竭触发微菌落解体。微菌落的分解是由T4P缩回主动驱动的。使用激光镊子，我们发现在有氧条件下，T4P-T4P相互作用力超过50 pN。在厌氧条件下，T4P-T4P相互作用被严重抑制。我们的结论是，氧是淋病球菌的微菌落形成所必需的加强毛-毛相互作用。
The formation of small bacterial clusters, called microcolonies, is the first step towards the formation of bacterial biofilms. The human pathogen Neisseria gonorrhoeae requires type IV pili (T4P) for microcolony formation and for surface motility. Here, we investigated the effect of oxygen on the dynamics of microcolony formation. We found that an oxygen concentration exceeding 3 mu M is required for formation and maintenance of microcolonies. Depletion of proton motive force triggers microcolony disassembly. Disassembly of microcolonies is actively driven by T4P retraction. Using laser tweezers we showed that under aerobic conditions T4P-T4P interaction forces exceed 50 pN. Under anaerobic conditions T4P-T4P interaction is severely inhibited. We conclude that oxygen is required for gonococcal microcolony formation by enhancing pilus-pilus interaction.