Fluorescent Gold Nanoclusters as a Biocompatible Marker for In Vitro and In Vivo Tracking of Endothelial Cells
Fluorescent Gold Nanoclusters as a Biocompatible Marker for In Vitro and In Vivo Tracking of Endothelial Cells
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DOI：
10.1021/nn102752a
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发表时间：
2011-06-01
期刊：
影响因子：
17.1
通讯作者：
Yeh, Hung-I
中科院分区：
文献类型：
--
作者：
Wang, Hsueh-Hsiao;Lin, Cheng-An J.;Yeh, Hung-I
本文研究了新型荧光金纳米团簇（FANC）在人主动脉内皮细胞（HAEC）和内皮祖细胞（EPC）中的荧光特性和生物相容性。FANC （50-1000 nmol/L）通过脂质体复合物进入细胞。荧光持续时间至少28天，体外半衰期为9天。对12个调节内皮细胞基本功能的转录本进行72h后的检测显示，在高浓度（500 nmol/L）下，只有血管细胞粘附分子1和血管内皮钙粘蛋白下调。此外，未检测到caspase 3或增殖细胞核抗原的活化。3[4,5-二甲基噻唑-2-酰基]-2,5-二苯基溴化四唑（MTT）试验表明，与量子点处理的细胞活力明显抑制不同，FANC对细胞活力的影响很小，除非超过500 nmol/L，在500 nmol/L以上，主要由脂质体引起的活力轻微降低被发现。成管实验显示，FANC处理后的EPC血管生成未受影响。对后肢缺血小鼠肌肉注射fnc标记的人EPC的体内研究表明，细胞在21天后保留了血管生成的潜力，并显示出可追踪的信号。这些发现表明，FANC是一种很有前途的生物相容性荧光探针。
We have been investigating the fluorescent property and biocompatibility of novel fluorescent gold nanoclusters (FANC) in human aortic endothelial cells (HAEC) and endothelial progenitor cells (EPC). FANC (50-1000 nmol/L) was delivered into cells via the liposome complex. The fluorescence lasted for at least 28 days with a half-life of 9 days in vitro. Examination of 12 transcripts regulating the essential function of endothelial cells after a 72 h delivery showed that only the vascular cell adhesion molecule 1 and the vascular endothelial cadherin were down-regulated at high concentration (500 nmol/L). In addition, no activation of caspase 3 or proliferating cell nuclear antigens was detected. 3[4,5-Dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide (MTT) assay demonstrated that, unlike the markedly suppressed viability in cells treated with quantum dots, FANC had minimal effect on the viability, unless above 500 nmol/L, at which level a minor reduction of viability mainly caused by liposome was found. Tube formation assay showed no Impaired angiogenesis in the EPC treated with FANC. In vivo study using hindlimb ischemic mice with an intramuscular injection of FANC-labeled human EPC showed that the cells preserved an angiogenic potential and exhibited traceable signals after 21 days. These findings demonstrated that FANC is a promising biocompatible fluorescent probe.