T cell progenitors emerge earlier than B cell progenitors in the murine fetal liver
T cell progenitors emerge earlier than B cell progenitors in the murine fetal liver
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DOI：
10.1016/s1074-7613(00)80196-x
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发表时间：
2000-04-01
期刊：
影响因子：
32.4
通讯作者：
Katsura, Y
中科院分区：
文献类型：
--
作者：
Kawamoto, H;Ikawa, T;Katsura, Y
使用能够确定祖细胞产生髓样、T和B细胞的潜力的克隆试验，研究了来自小鼠胎肝的Lin（-）c-kit（+）CD 45（+）IL-7 R（+）（IL-7 R（+））群体中单个细胞的发育潜力。在IL-7 R（+）群体中发现产生T细胞（p-T）或B细胞（p-B）的单能祖细胞，但未发现其他类型的祖细胞。在妊娠第12天，大部分祖细胞是p-T，而p-T的频率随胎龄显著降低。与此形成鲜明对比的是，p-B在第12天非常罕见，但此后迅速增加。这些发现有力地表明多能祖细胞向T和B细胞谱系的定向分化是独立发生的。
The developmental potential of individual cells in the Lin(-)c-kit(+)CD45(+)IL-7R(+) (IL-7R(+)) population from murine fetal liver was investigated using a clonal assay capable of determining the potential of a progenitor to give rise to myeloid, T, and B cells. Unipotent progenitors generating T cells (p-T) or B cells (p-B) but not other types of progenitors were found in the IL-7R(+) population. A large proportion of progenitors at day 12 of gestation are p-T, whereas the frequency of p-T dramatically decreases with gestational age. In marked contrast, p-B are very rare by day 12, but they rapidly increase thereafter. These findings strongly suggest that the commitment of multipotent progenitors to T and B cell lineages occurs independently.