Antibiofilm peptides as a promising strategy: comparative research
Antibiofilm peptides as a promising strategy: comparative research
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DOI：
10.1007/s00253-021-11103-6
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发表时间：
2021-01-21
影响因子：
5
通讯作者：
Lin, Huancai
中科院分区：
文献类型：
--
作者：
Li, Jing;Chen, Dongru;Lin, Huancai
生物被膜导致了大约65%的感染，这些感染很难治疗。因此，寻找有效的低细胞毒性的抗生物膜制剂是非常重要的。具有抗生物被膜活性的多肽被认为是很有前途的解决方案，其最小生物被膜抑制浓度(MBICs)低于其最低抑菌浓度(MICS)的多肽是有吸引力的。因此，我们对已报道的具有抗生物膜活性的多肽进行了系统的总结和分类。共有51个具有抗生物被膜活性的多肽被分为14类。将这14种代表性多肽的最低抑菌浓度和最低抑菌浓度分别与革兰氏阳性菌变形链球菌、革兰氏阴性菌铜绿假单胞菌和真菌白色念珠菌进行比较。6个有代表性的多肽(C5-胸腔杀菌素、C6-Pac-525、C9-蛋白质-1、C11-TetraF2W-RR、C13-WLBU2和C14-蜂毒素)对细菌和真菌均有抗菌作用，其中4个(C9-蛋白质-1、C11-TetraF2W-RR、C13-WLBU2和C14-蜂毒素)具有抑制生物被膜形成的作用，其MBIC值低于各自的MIC值。用激光共聚焦显微镜(CLSM)、扫描电子显微镜(SEM)和透射电子显微镜(TEM)进一步观察了多肽处理后生物膜的形态。在上述4种多肽中，WLBU2和蜂毒素在不破坏细菌的情况下稀疏地分散生物膜。综上所述，目前报道的具有抗生物被膜活性的多肽数量有限，但MBICs低于MICs的多肽仍有希望成为抗生物被膜相关感染的候选化合物，需要进一步研究。
Biofilms lead to approximately 65% of infections, and these infections are hard to treat. Thus, it is crucial to identify effective antibiofilm agents with low cytotoxicity. Peptides with antibiofilm activity have been regarded as promising solutions, and peptides with MBICs (minimal biofilm inhibitory concentrations) that are lower than their minimal inhibitory concentration (MICs) (minimal inhibitory concentrations) are appealing. Therefore, we systematically summarized and classified previously reported peptides with antibiofilm activity. A total of 51 peptides with antibiofilm activity were classified into 14 categories. The MICs and MBICs of these fourteen representative peptides, one selected from each category, were compared against the Gram-positive bacterium Streptococcus mutans, the Gram-negative bacterium Pseudomonas aeruginosa, and the fungus Candida albicans. Six representative peptides (C5-pleurocidin, C6-Pac-525, C9-protegrin-1, C11-TetraF2W-RR, C13-WLBU2, and C14-melittin) showed antibiofilm activity against both bacteria and fungi, and among these 6 representative peptides, 4 peptides (C9-protegrin-1, C11-TetraF2W-RR, C13-WLBU2, and C14-melittin) could prevent biofilm formation with lower MBIC values than their MICs. CLSM (confocal laser scanning microscopy), SEM (scanning electron microscopy), and TEM (transmission electron microscopy) were further used to observe the morphologies of the biofilms after treatment with the peptides. Among the above 4 peptides, WLBU2 and melittin sparsely scattered the biofilms without destroying the bacteria. In conclusion, the currently reported peptides with antibiofilm activity are limited in number, but peptides with lower MBICs than MICs exist as promising candidates against biofilm-related infections and need further study.