DETECTION AND CHARACTERIZATION OF INTERLEUKIN-1 IN HUMAN DENTAL PULPS
DETECTION AND CHARACTERIZATION OF INTERLEUKIN-1 IN HUMAN DENTAL PULPS
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DOI：
10.1016/0003-9969(89)90102-7
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发表时间：
1989-01-01
影响因子：
3
通讯作者：
LACHMAN, LB
中科院分区：
文献类型：
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作者：
DSOUZA, R;BROWN, LR;LACHMAN, LB
牙髓组织取自临床完整的第三磨牙和前磨牙，以及受龋齿和牙周病影响的牙齿。孵育和匀浆后，离心上清，取样，使用胸腺嘧啶结合的D10.G4.1细胞系测定淋巴细胞活化因子样活性。在有症状的龋齿和伴有冠周炎的第三磨牙的牙髓上清液中发现的有丝分裂活性水平明显高于有症状和无症状的牙周病牙齿以及无症状的第三磨牙和前磨牙的牙髓。白细胞介素-1 （IL-1）和白细胞介素-2 （IL-2）均可能参与D10的增殖。用小鼠自然杀伤（NK）细胞进一步检测龋牙髓G4.1细胞上清液的IL-2活性。引起D10.G4.1细胞增殖反应的细胞没有增强NK细胞的增殖，说明D10.G4.1细胞克隆复制的因子是IL-1。为了确认IL-1的存在，对各组纸浆的冷冻切片和石蜡包埋切片进行免疫组织化学，使用针对IL-1的单克隆抗体。IL-1免疫定位于龋症状组牙髓细胞内。产生IL-1的细胞类型进一步表征为龋症状组牙髓结缔组织基质中的α -萘乙酸酯酶阳性巨噬细胞。这项在人类牙髓中发现Il-1和产生Il-1细胞的新研究表明，这种炎症介质在牙病中的作用。
Pulp tissue was obtained from clinically intact third molars and premolars, and from teeth affected by caries and periodontal disease. After incubation and homogenization, supernatants were centrifuged, sampled and assayed for lymphocyte activating factor-like activity using the thymidine-incorporated D10.G4.1 cell-line assay. Significantly higher levels of mitogenic activity were found in the pulp supernatants of symptomatic carious teeth, and in those of third molars with attendant pericoronitis, than in pulps from symptomatic and asymptomatic periodontally affected teeth and asymptomatic third molars and premolars. As both interleukin-1 (IL-1) and interleukin-2 (IL-2) may be involved in the proliferation of D10. G4.1 cells supernatants from carious pulp were further tested for IL-2 activity using murine natural killer (NK) cells. Those that had elicited a proliferative response of D10.G4.1 cells did not enhance NK proliferation, indicating that the factor responsible for clonal replication of D10.G4.1 cells was IL-1. To confirm the presence of IL-1, immunohistochemistry using a monoclonal antibody to IL-1 was performed on frozen and paraffin-embedded sections of pulps from each group. IL-1 was immunolocalized within cells in pulps from the caries symptomatic group. The cell type producing IL-1 was further characterized as an alpha-naphthyl-acetate-esterase-positive macrophage within the connective tissue stroma of pulps from the caries symptomatic group. This novel demonstration of Il-1 and IL-1 producing cells in human dental pulp indicates the involvement of this mediator of inflammation in dental disease.