Role of cytokinin responsive two-component system in ABA and osmotic stress signalings
Role of cytokinin responsive two-component system in ABA and osmotic stress signalings
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DOI：
10.4161/psb.5.2.10411
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Yamaguchi-Shinozaki, Kazuko
中科院分区：
文献类型：
--
作者：
Lam-Son Phan Tran;Shinozaki, Kazuo;Yamaguchi-Shinozaki, Kazuko
双组分信号系统（two-component signaling systems，TCS）介导组氨酸-天冬氨酸信号通路，控制着许多生物体的多种生物学过程，包括细胞分裂、细胞生长和增殖以及对环境刺激和生长调节剂的反应。我们在植物中提供了细胞分裂素（CK）反应性TCS介导脱落酸（ABA）和渗透胁迫反应的证据。利用功能丧失的方法，我们证明了三种细胞分裂素（CK）受体组氨酸激酶AHK2、AHK3和AHK4/CRE1在ABA、干旱和高盐胁迫信号中起负调节作用。抗逆<ahk2，3>双突变体的全基因组表达谱表明CK受体激酶以ABA依赖和ABA非依赖的方式介导渗透胁迫反应。此外，我们发现CK在介导应激反应中的作用的证据，从AHK 4需要CK在渗透应激反应中起负调节作用的事实判断。研究结果表明，CK、ABA和渗透胁迫信号通路之间存在着相互作用，CK信号通路和CK代谢不仅在植物生长发育过程中起着重要作用，而且在渗透胁迫信号通路中也起着重要作用。
The two-component signaling systems (TCSs), which mediate the histidine-aspartate signaling, control diverse biological processes of many organisms, including cell division, cell growth and proliferation and responses to environmental stimuli and growth regulators. We have provided in planta evidence that the cytokinin (CK) responsive TCS mediates abscisic acid (ABA) and osmotic stress responses. By using loss-of-function approach we have demonstrated that the three cytokinin (CK) receptor histidine kinases AHK2, AHK3 and AHK4/CRE1 act as negative regulators in ABA, drought and high salinity stress signalings. Genome-wide expression profiling of the stress-tolerant < ahk2,3 > double mutant suggested that CK receptor kinases mediate osmotic stress response in both an ABA-dependent and ABA-independent manner. Additionally, we showed evidence for the role of CK in mediating stress responses, judging from the fact that AHK4 requires the CK to function as a negative regulator in osmotic stress response. Our results suggested that cross-talk exists among CK, ABA and osmotic stress signaling pathways, and that CK signaling and CK metabolism may play crucial roles not only in plant growth and development but also in osmotic stress signaling.