Use of intrinsic optical signals to monitor physiological changes in brain tissue slices
Use of intrinsic optical signals to monitor physiological changes in brain tissue slices
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DOI：
10.1006/meth.1999.0762
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发表时间：
1999-06-01
期刊：
影响因子：
4.8
通讯作者：
Turner, DA
中科院分区：
文献类型：
--
作者：
Aitken, PG;Fayuk, D;Turner, DA
光学成像技术有可能为脑功能研究带来高空间和时间分辨率的组合。许多光学技术需要在组织中添加染料或荧光标记，并且这些方法已被证明是非常有价值的。还已知神经组织的固有光学特性受到某些生理变化的影响，并且这些固有光学信号可以提供通过其他手段不可用的信息。大多数作者将内在光学变化归因于细胞体积的改变和胞质溶胶浓度的伴随变化。在这篇文章中，我们回顾了有关内在光信号的文献，涵盖了光学变化的机制及其在神经生理学各个分支中的应用。我们还讨论了技术方面的技术与海马切片，包括照明方法，相机，实验方法，数据收集和分析程序。最后，我们提出的数据调查中，我们使用的内在光学信号在海马切片研究的范围内的突触激活，传播扩散性抑郁症，缺氧的程度和严重程度的反应，和组织的渗透压的挑战。我们的结论是：（1）海马脑片至少有两个过程产生内源性光信号，其中一个过程引起光散射与细胞体积成反比，与细胞质稀释有关，另一个过程可能与线粒体肿胀有关;以及（2）固有光信号可以是用于相对慢的事件的空间映射的有用工具，但不适合于快速生理过程的研究。(C)北京：科学出版社.
Optical imaging techniques have the potential to bring a combination of high spatial and temporal resolution to studies of brain function. Many optical techniques require the addition of a dye or fluorescent marker to the tissue, and such methods have proven extremely valuable. It is also known that the intrinsic optical properties of neural tissue are affected by certain physiological changes and that these intrinsic optical signals can provide information not available by other means. Most authors attribute the intrinsic optical change to alterations in cell volume and concomitant change in the concentration of the cytosol. In this article we review the literature on intrinsic optical signals, covering both the mechanisms of the optical change and its use in various branches of neurophysiology. We also discuss technical aspects of the technique as used with hippocampal slices, including illumination methods, cameras, experimental methods, and data collection and analysis procedures. Finally we present data from investigations in which we used intrinsic optical signals in hippocampal slices to study the extent of spread of synaptic activation, propagation of spreading depression, extent and severity of the response to hypoxia, and tissue response to osmotic challenges. We conclude that (1) at least two processes generate intrinsic optical signals in hippocampal slices, one of which causes light scattering to change inversely with cell volume and is related to dilution of the cytoplasm, while the other, opposite in sign, may be due to mitochondrial swelling; and (2) the intrinsic optical signal can be a useful tool for spatial mapping of relatively slow events, but is not suitable for study of fast physiological processes. (C) 1999 Academic Press.