Decoupling from yolk sac is required for extraembryonic tissue spreading in the scuttle fly Megaselia abdita
Decoupling from yolk sac is required for extraembryonic tissue spreading in the scuttle fly Megaselia abdita
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DOI：
10.7554/elife.34616.001
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发表时间：
2018-10-30
期刊：
影响因子：
7.7
通讯作者：
Lemke, Steffen
中科院分区：
文献类型：
--
作者：
Carot, Francesca;Avalos, Everardo Gonzalez;Lemke, Steffen
胚外组织有助于动物的发育，这通常需要扩展到胚胎或蛋黄上。除了细胞形状的变化，这种组织扩展的要求还没有得到很好的理解。在这里，我们分析了扩展的胚外浆膜在飞蝗abdita。浆膜由柱状胚盘原基形成，变成鳞状上皮，并最终覆盖整个胚胎。我们在长期的全胚胎延时记录中描述了这一过程的动力学，证明了组织扩张中的长时间暂停是游离浆膜扩散的前提。对这一停顿的更仔细的检查揭示了与下面的卵黄囊的机械耦合，卵黄囊随后被释放。我们发现，机械耦合延长和浆膜扩散受损后，敲低M。基质金属蛋白酶1.我们的结论是，组织-组织的相互作用提供了一个关键的功能元素，以限制蔓延的上皮细胞。
Extraembryonic tissues contribute to animal development, which often entails spreading over embryo or yolk. Apart from changes in cell shape, the requirements for this tissue spreading are not well understood. Here, we analyze spreading of the extraembryonic serosa in the scuttle fly Megaselia abdita. The serosa forms from a columnar blastoderm anlage, becomes a squamous epithelium, and eventually spreads over the embryo proper. We describe the dynamics of this process in long-term, whole-embryo time-lapse recordings, demonstrating that free serosa spreading is preceded by a prolonged pause in tissue expansion. Closer examination of this pause reveals mechanical coupling to the underlying yolk sac, which is later released. We find mechanical coupling prolonged and serosa spreading impaired after knockdown of M. abdita Matrix metalloprotease 1. We conclude that tissue-tissue interactions provide a critical functional element to constrain spreading epithelia.