Distinct profiles of immunoglobulin G-binding-protein expression by invasive serotype M1 isolates of Streptococcus pyogenes.
Distinct profiles of immunoglobulin G-binding-protein expression by invasive serotype M1 isolates of Streptococcus pyogenes.
复制标题
化脓性链球菌侵袭性血清型 M1 分离株免疫球蛋白 G 结合蛋白表达的独特特征。
DOI：
10.1128/cdli.2.4.478-483.1995
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Boyle,MD
中科院分区：
文献类型：
--
作者：
Raeder,R;Boyle,MD
作为疾病控制和预防中心监测研究的一部分收集的侵袭性A组链球菌M1血清型分离株的免疫球蛋白G（IgG）结合蛋白表达分析显示了两种不同的表型。一组M1型分离株表达与所有四种人IgG亚类（IIo型）反应的表面蛋白，而第二组表达仅与人IgG 3（IIb型）具有显著反应性的表面蛋白。IgG结合蛋白的功能形式是抗原相关的，两者都被兔多克隆抗血清M1血清型，但不是由正常兔血清识别。虽然呈现每种表型的代表性分离株提取物中存在的抗原性M1蛋白的量不同，但发现功能差异是定性的，而不仅仅是定量的。这些抗原相关的M1蛋白的IgG结合特性可以容易地与另一种IgG结合蛋白（蛋白H）的IgG结合特性区分开。M1型分离株的IIb表型不同于IIo表型分泌大量的酪蛋白水解蛋白酶到培养上清液。
Analysis of immunoglobulin G (IgG)-binding-protein expression by invasive group A streptococcal isolates of the M1 serotype collected as part of a Centers for Disease Control and Prevention surveillance study revealed two distinct phenotypes. One group of type M1 isolates expressed a surface protein reactive with all four human IgG subclasses (type IIo), while a second group expressed a surface protein demonstrating significant reactivity only with human IgG3 (type IIb). The functional forms of IgG-binding protein were antigenically related, and both were recognized by a rabbit polyclonal antiserum to serotype M1 but not by normal rabbit serum. While the quantities of antigenic M1 protein present in the extracts of representative isolates displaying each phenotype differed, the functional differences were found to be qualitative and not solely quantitative. The IgG-binding properties of these antigenically related M1 proteins could be readily distinguished from those of another IgG-binding protein, protein H. Type M1 isolates of the IIb phenotype differed from those of the IIo phenotype by secreting larger amounts of a casein-hydrolyzing protease into culture supernatants.