EVIDENCE FOR A CHROMOSOME-BORNE RESISTANCE TRANSPOSON (TN916) IN STREPTOCOCCUS-FAECALIS THAT IS CAPABLE OF CONJUGAL TRANSFER IN THE ABSENCE OF A CONJUGATIVE PLASMID
EVIDENCE FOR A CHROMOSOME-BORNE RESISTANCE TRANSPOSON (TN916) IN STREPTOCOCCUS-FAECALIS THAT IS CAPABLE OF CONJUGAL TRANSFER IN THE ABSENCE OF A CONJUGATIVE PLASMID
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DOI：
10.1128/jb.145.1.494-502.1981
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发表时间：
1981-01-01
影响因子：
3.2
通讯作者：
CLEWELL, DB
中科院分区：
文献类型：
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作者：
FRANKE, AE;CLEWELL, DB
S.粪肠球菌菌株DS 16含有决定对链霉素、卡那霉素和红霉素的抗性的接合性溶血素-杆菌素质粒pAD 1（35兆道尔顿）和非接合性R质粒pAD 2;四环素抗性（Tetr）决定子位于染色体上。当菌株DS 16与无质粒菌株JH 2 -2交配时（在膜滤器上），Tetr接合子可以以约10-6/受体的频率获得。对接合子的分析表明，一些接合子含有与pAD 1相连的Tetr决定簇。随后的研究表明Tetr决定簇位于10 M道尔顿的转座子上，命名为Tn 916，其可以插入到2个其它溶血素质粒：pAM γ 1和pOB 1中。没有pAD 1的DS 16衍生物能够将Tetr转移到受体菌株。从这样的交配Transconjugants（无质粒）可以随后作为捐助者在转移Tetr。转座和转移不依赖于受体。
S. faecalis strain DS16 harbors the conjugative hemolysin-bacterocin plasmid pAD1 (35 megadaltons) and the nonconjugative R-plasmid pAD2 determining resistance to streptomycin, kanamycin and erythromycin; a tetracycline resistance (Tetr) determinant is located on the chromosome. When strain DS16 was mated (on membrane filters) with the plasmid-free strain JH2-2, Tetr transconjugants could be obtained at a frequency of about 10-6/recipient. Analyses of transconjugants showed that some contained the Tetr determinant linked to pAD1. Subsequent studies showed that the Tetr determinant was located on a 10 Mdalton transposon, designated Tn916, which could insert into 2 other hemolysin plasmids: pAM.gamma.1 and pOB1. Derivatives of DS16 devoid of pAD1 were capable of transferring Tetr to recipient strains. Transconjugants (plasmid-free) from such matings could subsequently act as donors in the transfer of Tetr. Transposition and transfer were rec independent.