Subarachnoid hemorrhage-induced hydrocephalus in rats.
Subarachnoid hemorrhage-induced hydrocephalus in rats.
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DOI：
10.1161/strokeaha.112.662312
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发表时间：
2013-02
期刊：
影响因子：
8.3
通讯作者：
Xi G
中科院分区：
文献类型：
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作者：
Okubo S;Strahle J;Keep RF;Hua Y;Xi G
脑积水是蛛网膜下腔出血（SAH）的重要并发症。我们在大鼠SAH模型中研究了急性脑积水的发生。在成年雄性Sprague-Dawley大鼠（n=36）中通过血管内穿孔诱导SAH。假手术大鼠（n=8）进行相同的程序，没有穿孔。SAH后24小时进行MRI，测量脑室系统的体积和T2* 低信号病变的范围。我们将脑积水定义为假手术动物的脑室容积高于平均值+3标准差。测定SAH分级，并将脑用于组织学、免疫组织化学、Perls染色和Western印迹分析。室壁损伤定义为室管膜表面破裂的百分比。SAH后所有存活大鼠（n=27）均出现心室扩大（33.6±4.7 vs.假手术动物中的13.5±1.4 mm 3，p<0.01）。心室容积与SAH严重程度相关（r=0.48，p<0.05）。27只SAH大鼠中有12只表现为脑积水，且均存在脑室内积血。脑积水组大鼠心室壁损伤（7.4±1.2%）明显高于假手术组（0.6± 0.2%，p<0.01）和无脑积水组（1.1± 0.2%，p<0.01）。脑积水大鼠脑室周围可见铁沉积，HO-1和Iba-1表达明显增加。在大鼠血管内穿孔模型中，SAH导致脑室扩大，24小时时44%的动物发生脑积水。脑积水组大鼠SAH、脑室出血及脑室壁损伤程度较对照组重。
Hydrocephalus is an important complication of subarachnoid hemorrhage (SAH). We investigated the occurrence of acute hydrocephalus in a rat SAH model. SAH was induced by endovascular perforation in adult male Sprague-Dawley rats (n=36). Sham rats (n=8) underwent the same procedure without perforation. MRI was performed 24 hours after SAH and the volume of the ventricular system and extent of T2* hypointensity lesions were measured. We defined hydrocephalus as ventricular volume greater than +3 standard deviations above the mean in sham animals. SAH grade was determined and brains were used for histology, immunohistochemistry, Perls’ staining and Western blot analysis. Ventricular wall damage was defined as percentage of ependymal surface disruption. All surviving rats (n=27) after SAH had ventricular enlargement (33.6±4.7 vs. 13.5±1.4 mm3 in sham animals, p<0.01). Ventricular volume correlated with SAH severity (r=0.48, p<0.05). 12 of 27 SAH rats demonstrated hydrocephalus and all had intraventricular blood accumulation. Rats with hydrocephalus had more severe ventricular wall damage (7.4±1.2%) than the sham animals (0.6±0.2%, p<0.01) and rats without hydrocephalus (1.1±0.2%, p<0.01). Periventricular iron deposition was observed and HO-1 and Iba-1 expression were markedly increased in hydrocephalus rats. SAH causes ventricular enlargement in a rat endovascular perforation model, with hydrocephalus occurring in 44% of animals at 24 hours. Rats with hydrocephalus had more severe SAH, intraventricular hemorrhage and greater ventricular wall damage.