Morphine-induced place preference: Involvement of cholinergic receptors of the ventral tegmental area
Morphine-induced place preference: Involvement of cholinergic receptors of the ventral tegmental area
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DOI：
10.1016/j.ejphar.2007.01.081
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发表时间：
2007-05-07
影响因子：
5
通讯作者：
Delphi, Ladan
中科院分区：
文献类型：
--
作者：
Rezayof, Ameneh;Nazari-Serenjeh, Farzaneh;Delphi, Ladan
在本研究中，采用无偏3天的位置条件反射设计，研究了腹侧被盖区注射胆碱能药物对吗啡诱导的条件位置偏好的影响。皮下注射吗啡（0.5 ~ 7.5 mg/kg）对药物相关部位产生了明显的剂量依赖性条件部位偏好。Intra-ventral盖的区域注入抗胆碱酯酶,毒扁豆碱(2.5和5μg /鼠)或烟碱乙酰胆碱受体激动剂,尼古丁(0.5和1μg /鼠)与一个无效的剂量的吗啡(0.5毫克/公斤)引起了重要的条件性位置偏爱。此外，毒蕈碱类乙酰胆碱受体拮抗剂阿托品（1-4 μ g/大鼠）或烟碱类乙酰胆碱受体拮抗剂甲胺（5和7.5 μ g/大鼠）在被盖区内给予剂量依赖性抑制吗啡（5 mg/kg）诱导的部位偏好。阿托品和甲胺分别逆转了毒豆碱和尼古丁对吗啡反应的影响。在腹侧被盖区单独注射毒豆碱，而不是阿托品、尼古丁或甲胺，会产生明显的地方厌恶。此外，腹侧被盖区内给予高剂量的毒豆碱或阿托品，而不是尼古丁或甲胺，可以降低运动活动。我们认为腹侧被盖区的毒蕈碱和烟碱乙酰胆碱受体可能介导吗啡的奖赏效应。(c) 2007 Elsevier B.V.版权所有
In the present study, the effects of intra-ventral tegmental area injections of cholinergic agents on morphine-induced conditioned place preference were investigated by using an unbiased 3-day schedule of place conditioning design in rats. The conditioning treatments with subcutaneous injections of morphine (0.5-7.5 mg/kg) induced a significant dose-dependent conditioned place preference for the drug-associated place. Intra-ventral tegmental area injection of an anticholinesterase, physostigmine (2.5 and 5 mu g/rat) or nicotinic acetylcholine receptor agonist, nicotine (0.5 and 1 mu g/rat) with an ineffective dose of morphine (0.5 mg/kg) elicited a significant conditioned place preference. Furthermore, intraventral tegmental area administration of muscarinic acetylcholine receptor antagonist, atropine (1-4 mu g/rat) or nicotinic acetylcholine receptor antagonist, mecamylamine (5 and 7.5 mu g/rat) dose-dependently inhibited the morphine (5 mg/kg)-induced place preference. Atropine or mecamylamine reversed the effect of physostigmine or nicotine on morphine response respectively. The injection of physostigmine, but not atropine, nicotine or mecamylamine, into the ventral tegmental area alone produced a significant place aversion. Moreover, intra-ventral tegmental area administration of the higher doses of physostigmine or atropine, but not nicotine or mecamylamine decreased the locomotor activity. We conclude that muscarinic and nicotinic acetylcholine receptors in the ventral tegmental area may critically mediate the rewarding effects of morphine. (c) 2007 Elsevier B.V. All rights reserved.