PALD encoding a lipid droplet-associated protein is critical for the accumulation of lipid droplets and pollen longevity in Arabidopsis
PALD encoding a lipid droplet-associated protein is critical for the accumulation of lipid droplets and pollen longevity in Arabidopsis
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DOI：
10.1111/nph.18123
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发表时间：
2022-04-12
期刊：
影响因子：
9.4
通讯作者：
Gao, Xin-Qi
中科院分区：
文献类型：
--
作者：
Li, Fei;Han, Xiao;Gao, Xin-Qi
花粉寿命对植物授粉和杂交制种至关重要，但很少有人关注花粉寿命。在这项研究中，我们鉴定了拟南芥的一个基因，与脂滴相关的蛋白(PALD)，它在花粉中强烈表达，对花粉寿命的调节至关重要。PALD在成熟花粉粒和花粉管中特异表达，在干燥条件下表达上调。PALD编码一种脂滴相关蛋白，其N末端对脂滴的定位起着至关重要的作用。功能缺失的PALD突变体的花粉粒中LDs数目减少，直径减小。Pald突变体的成熟花粉活力和萌发率与野生型相当，但在干燥条件下保存后，突变体的花粉萌发和雄性传递与野生型相比受到影响。我们的研究表明，PALD在维持成熟花粉粒的LD质量和调节花粉寿命方面是必需的。
Pollen longevity is critical for plant pollination and hybrid seed production, but few studies have focused on pollen longevity. In this study, we identified an Arabidopsis thaliana gene, Protein associated with lipid droplets (PALD), which is strongly expressed in pollen and critical for the regulation of pollen longevity. PALD was expressed specifically in mature pollen grains and the pollen tube, and its expression was upregulated under dry conditions. PALD encoded a lipid droplet (LD)-associated protein and its N terminus was critical for the LD localization of PALD. The number of LDs and diameter were reduced in pollen grains of the loss-of-function PALD mutants. The viability and germination of the mature pollen grains of the pald mutants were comparable with those of the wild-type, but after the pollen grains were stored under dry conditions, pollen germination and male transmission of the mutant were compromised compared with those of the wild-type. Our study suggests that PALD was required for the maintenance of LD quality in mature pollen grains and regulation of pollen longevity.