Functional analysis of four Gloverin-like genes in the silkworm, Bombyx mori
Functional analysis of four Gloverin-like genes in the silkworm, Bombyx mori
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DOI：
10.1002/arch.20223
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发表时间：
2008-02-01
影响因子：
2.2
通讯作者：
Shimada, Toru
中科院分区：
文献类型：
--
作者：
Kawaoka, Shinpei;Katsuma, Susumu;Shimada, Toru
为了鉴定家蚕先天免疫相关基因，我们从大肠埃希氏菌家蚕幼虫的脂肪体中构建了cDNA文库。基于表达序列标签（EST）数据和全基因组霰弹枪序列分析，我们在家蚕基因组中发现了4个gloverin样基因BmGlov1-4。Northern blot和RTPCR分析表明，在大肠杆菌免疫攻击后，BmGlov1-4在幼虫脂肪体中被诱导，而在白色念珠菌免疫攻击后，BmGlov1-4的诱导作用较弱。硅基序列分析显示，在每个BmGlov基因的上游区域存在一个与NF-kappa B结合位点同源的基序。此外，我们利用杆状病毒表达系统表达了重组BmGlov1-4蛋白，发现所有重组BmGlov1-4蛋白都能显著抑制大肠杆菌的生长。
To identify genes involved in the innate immunity of the silkworm Bombyx mori, we constructed a cDNA library from the fat body of Escherichia coli-challenged B. mori larvae. Based on the expressed sequence tag (EST) data and whole genome shotgun sequence analysis, we found four Gloverin-like genes, BmGlov1-4, in the Bombyx genome. Northern blot and RTPCR analysis showed that BmGlov1-4 were induced in the larval fat body after an immune challenge by the injection of E. coli, however, less induction was observed after the injection of a yeast Candida albicons. In silico sequence analysis revealed the presence of a motif homologous to NF-kappa B binding site in the upstream region of each BmGlov gene. Moreover, we expressed recombinant BmGlov1-4 proteins using the baculovirus expression system, and found that all the recombinant BmGlov1-4 significantly inhibited the growth of E. coli.