Detection of quantitative trait loci for phosphorus deficiency tolerance at soybean seedling stage
Detection of quantitative trait loci for phosphorus deficiency tolerance at soybean seedling stage
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DOI：
10.1007/s10681-009-9880-0
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发表时间：
2009-01
期刊：
影响因子：
1.9
通讯作者：
Dan Zhang;Hao Cheng;Leiyue Geng;Guizhen Kan;S. Cui;Q. Meng;J. Gai;Deyue Yu
中科院分区：
文献类型：
--
作者：
Dan Zhang;Hao Cheng;Leiyue Geng;Guizhen Kan;S. Cui;Q. Meng;J. Gai;Deyue Yu
缺磷是酸性和石灰性土壤上大豆生产的主要限制因素。以耐磷品种南农94 -156和磷敏感品种博高杂交获得的152个重组自交系为材料，对耐低磷的QTL进行定位。2005年和2006年，在不同磷水平下，以5个性状作为苗期耐低磷性的评价指标。在9个连锁群上共检测到34个加性QTL，贡献率为6.6- 19.3%。在基因组区域S506-Satt 534（连锁群B2-1）、Sat_183-Satt 274（连锁群D1 b + W）和Sat_185-Satt 012（连锁群G）中发现3个QTL簇。在D1 b + W连锁群上位于Sat_183-Satt 274之间的位点与4个已发现的磷效率QTL一致。另一个有趣的位点两侧的Sat_185-Satt 012连锁群G上检测到跨年。该QTL的发现将有助于提高大豆对缺磷引起的复杂营养失调的抗性。此外，在低磷条件下检测到较多的QTL，并检测到一些在不同磷水平下特异表达的QTL。这些特异的QTL有助于更好地理解大豆磷效率的遗传基础。
Phosphorus deficiency is a primary constraint to soybean productivity in acid and calcareous soils. Our aim was to map quantitative trait loci (QTL) controlling phosphorus deficiency tolerance using 152 recombinant inbred lines derived from a cross between the P stress tolerant variety Nannong94-156 and the P stress sensitive variety Bogao. Five traits were used as parameters to evaluate phosphorus deficiency tolerance at seedling stage under different phosphorus levels in experiments 2005 and 2006. As a result, thirty-four additive QTLs were detected on nine linkage groups, with corresponding contribution ratios of 6.6–19.3%. There were three clusters of QTL found in genomic regions S506-Satt534 (on linkage group B2-1), Sat_183-Satt274 (on linkage group D1b + W), and Sat_185-Satt012 (on linkage group G). The locus flanked by Sat_183-Satt274 on linkage group D1b + W was coincident with four previously discovered QTLs with phosphorus efficiency. Another interesting locus flanked by Sat_185-Satt012 on linkage group G was detected across years. The identified QTL will be useful to improve the stress resistance of soybean against a complex nutritional disorder caused by phosphorus deficiency. In addition, more QTLs were detected under low phosphorus condition and some QTLs were detected that specifically expressed under different phosphorus levels. These particular QTLs could help provide greater understanding of the genetic basis of phosphorus efficiency in soybean.