A study of the parameters affecting flow gradient analysis of catecholamines, DOPA and DOPAC by ion pair liquid chromatography with electrochemical detection.
A study of the parameters affecting flow gradient analysis of catecholamines, DOPA and DOPAC by ion pair liquid chromatography with electrochemical detection.
复制标题
离子对液相色谱电化学检测影响儿茶酚胺、DOPA 和 DOPAC 流量梯度分析的参数研究。
DOI：
10.1016/0024-3205(83)90632-x
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
C. A. Rivano
中科院分区：
文献类型：
--
作者：
Z. Rossetti;G. Mercuro;C. A. Rivano
本文介绍了一种测定去甲肾上腺素、肾上腺素、多巴胺、3，4-二羟基苯丙氨酸和3，4-二羟基苯乙酸的通用方法。它利用离子对反相液相色谱分析过程中产生流动梯度。该方法允许减少分析时间，保持接近溶剂前沿的峰的高分辨率。评价了流动梯度对柱效、检测器响应和保留的影响。羊水，尿液，人血浆和大鼠脑组织标本的应用程序。
A general method for the determination of norepinephrine, epinephrine, dopamine, 3,4-dihydroxyphenylalanine and 3,4-dihydroxyphenylacetic acid is described. It employes the generation of a flow gradient during the analysis by ion-pair reversed phase liquid chromatography. The method allows a reduction of the analysis time maintaining high resolution of the peaks close to the solvent front. The effect of the flow gradient on column efficiency, detector response and retention are evaluated. The application to specimens of amniotic fluid, urine, human plasma and rat brain tissue is shown.