Selection of mitotic Chinese hamster ovary cells from microcarriers.
Selection of mitotic Chinese hamster ovary cells from microcarriers.
复制标题
从微载体中选择有丝分裂的中国仓鼠卵巢细胞。
DOI：
10.1016/0003-2697(80)90641-7
复制
发表时间：
1980
影响因子：
2.9
通讯作者：
Thilly,WG
中科院分区：
文献类型：
--
作者：
Ng,JJ;Crespi,CL;Thilly,WG
我们描述了一种从悬浮培养的中国仓鼠卵巢细胞群中大量收集有丝分裂细胞的方法，这些细胞在带正电的葡聚糖微载体上呈指数增长。这些细胞用秋水仙素处理2.5h，通过提高旋转速度从微载体中获得有丝分裂细胞。用这种方法可获得总产量的2-3%，经显微镜观察，收集的细胞中有85%-95%处于中期。在选定的群体中，同步性和细胞存活率都很好。
We describe a method of collecting large quantities of mitotic cells from a population of Chinese hamster ovary cells which were exponentially growing on positively charged dextran microcarriers in suspension culture. These cells were treated for 2.5 h with colcemid, and mitotic cells were harvested from the oicrocarriers by increasing spinner velocity. A yield of 2–3% of the total population was obtained using this method; of the cells collected, 85–95% were in metaphase as determined by microscopic inspection. Both synchrony and cell viability were excellent in the selected population.