Timing of Fetal Meconium Absorption by Amnionic Macrophages
Timing of Fetal Meconium Absorption by Amnionic Macrophages
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DOI：
10.1055/s-0028-1103028
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发表时间：
2009-01-01
影响因子：
2
通讯作者：
Kliman, Harvey J.
中科院分区：
文献类型：
--
作者：
Funai, Edmund F.;Labowsky, Anna T.;Kliman, Harvey J.
本研究旨在确定从胎儿排便到膜染色和随后胎粪被巨噬细胞摄取的时间间隔。将外部膜片固定到特氟隆环上以形成孔。胎粪表面暴露于胎粪和羊水1至48小时，并在显微镜下分析游离胎粪和胎粪负载的胎粪巨噬细胞的存在。在每个实验中，在12小时时间点之前，膜层中存在少量装载胎粪的巨噬细胞。在24小时和48小时，在膜中观察到载有胎粪的巨噬细胞数量显著增加，同时染色强度增加。与以前的报道相反，我们的胎粪掺入胎盘膜的体外模型表明，只有在24和48小时后才观察到大量的胎粪负载的巨噬细胞。
This study sought to determine the time interval from fetal defecation to membrane staining and subsequent meconium uptake by amnionic macrophages. Pieces of external membranes were fastened to Teflon rings to create a well. The amnionic surface was exposed to meconium and amniotic fluid for 1 to 48 hours and analyzed microscopically for the presence of free meconium and meconium-laden amnionic macrophages. In each of the experiments, prior to the 12-hour time point, few meconium-laden macrophages were present in the membrane layers. A significant rise in the number of meconium-laden macrophages and a concomitant increase in staining intensity was noted in the membranes at 24 and 48 hours. Contrary to previous reports, our in vitro model of meconium incorporation into placental membranes demonstrated that significant numbers of meconium-laden macrophages were only observed after 24 and 48 hours.