THE HUMAN 180-KDA RECEPTOR FOR SECRETORY PHOSPHOLIPASES A(2) - MOLECULAR-CLONING, IDENTIFICATION OF A SECRETED SOLUBLE FORM, EXPRESSION, AND LOCALIZATION
THE HUMAN 180-KDA RECEPTOR FOR SECRETORY PHOSPHOLIPASES A(2) - MOLECULAR-CLONING, IDENTIFICATION OF A SECRETED SOLUBLE FORM, EXPRESSION, AND LOCALIZATION
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DOI：
10.1074/jbc.270.15.8963
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发表时间：
1995-04-14
影响因子：
4.8
通讯作者：
LAZDUNSKI, M
中科院分区：
文献类型：
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作者：
ANCIAN, P;LAMBEAU, G;LAZDUNSKI, M
分泌型磷脂酶A（2）（sPLA（2））是在哺乳动物以及昆虫和蛇毒中发现的结构相关的酶。它们与几种生理、病理和毒性过程有关。其中一些作用显然与毒液和哺乳动物sPLA（2）的特异性受体的存在有关。本文报道了人肾sPLA（2）受体的分子克隆和表达。检测到两个转录本。一个编码sPLA（2）受体的跨膜形式，另一个是替代加工的转录物，由转录单位C末端部分内含子内的位点处发生的聚腺苷酸化引起。该转录本编码一种缩短的分泌型可溶性sPLA（2）受体，缺乏跨膜片段的编码区。定量聚合酶链反应实验表明，编码膜结合型和可溶性形式受体的转录本水平之间的比例分别为1.6：1。可溶性和膜结合的人sPLA（2）受体都以高亲和力结合sPLA（2）。然而，人受体的结合特性与兔膜结合sPLA（2）受体的结合特性不同。180 kDa人sPLA（2）受体基因已定位于2号染色体q23-q24带。
Secretory phospholipases A(2) (sPLA(2)) are structurally related enzymes found in mammals as well as in insect and snake venoms. They have been associated with several physiological, pathological, and toxic processes. Some of these effects are apparently linked to the existence of specific receptors for both venom and mammalian sPLA(2)s. We report here the molecular cloning and expression of one of these sPLA(2) receptors from human kidney. Two transcripts were detected. One encodes for a transmembrane form of the sPLA(2) receptor and the other one is an alternatively processed transcript, caused by polyadenylation occurring at a site within an intron in the C terminus part of the transcriptional unit. This transcript encodes for a shortened secreted soluble sPLA(2) receptor lacking the coding region for the transmembrane segment, Quantitative polymerase chain reaction experiments indicate a 1.6:1 ratio between the levels of transcripts encoding for the membrane bound and soluble forms of the receptor, respectively. Soluble and membrane bound human sPLA(2) receptors both bind sPLA(2) with high affinities. However, the binding properties of the human receptors are different from those obtained with the rabbit membrane-bound sPLA(2) receptor. The 180 kDa human sPLA(2) receptor gene has been mapped in the q23-q24 bands of chromosome 2.