Chemical genetic screening identifies a novel inhibitor of parallel alignment of cortical microtubules and cellulose microfibrils
Chemical genetic screening identifies a novel inhibitor of parallel alignment of cortical microtubules and cellulose microfibrils
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DOI：
10.1093/pcp/pcm120
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发表时间：
2007-10-01
影响因子：
4.9
通讯作者：
Matsui, Minami
中科院分区：
文献类型：
--
作者：
Yoneda, Arata;Higaki, Takumi;Matsui, Minami
众所周知的假设是，皮质微管控制纤维素微纤维沉积的方向，并且平行的纤维素微纤维决定各向异性细胞扩张和植物细胞形态发生。然而，皮质微管调节纤维素微纤维取向的分子机制仍不清楚。为了研究这一机制，进行了化学遗传筛选。通过这次筛选，鉴定出 SS 化合物可诱导烟草 BY-2 细胞出现球形肿胀表型。 SS 化合物可分为三类：破坏皮质微管的化合物；破坏皮质微管的化合物；破坏皮质微管的化合物；破坏皮质微管的化合物。那些减少纤维素微纤维含量的；第三种是没有这些效果的。在最后一类中，发现一种名为 cobtorin 的化学物质在最低浓度下可诱导球形膨胀表型，表明与假定靶点具有很强的结合活性。使用紫杉醇处理的原生质体检查纤维素微纤维的再生情况表明，cobtorin 化合物扰乱了预先存在的皮质微管和新生纤维素微纤维的平行排列。因此，cobtorin 可能是一种新型抑制剂，也是进一步研究皮质微管引导纤维素微纤维平行沉积机制的有吸引力的工具。
It is a well-known hypothesis that cortical microtubules control the direction of cellulose microfibril deposition, and that the parallel cellulose microfibrils determine anisotropic cell expansion and plant cell morphogenesis. However, the molecular mechanism by which cortical microtubules regulate the orientation of cellulose microfibrils is still unclear. To investigate this mechanism, chemical genetic screening was performed. From this screening, SS compounds were identified that induced a spherical swelling phenotype in tobacco BY-2 cells. The SS compounds could be categorized into three classes: those that disrupted the cortical microtubules; those that reduced cellulose microfibril content; and thirdly those that had neither of these effects. In the last class, a chemical designated cobtorin was found to induce the spherical swelling phenotype at the lowest concentration, suggesting strong binding activity to the putative target. Examining cellulose microfibril regeneration using taxol-treated protoplasts revealed that the cobtorin compound perturbed the parallel alignment of pre-existing cortical microtubules and nascent cellulose microfibrils. Thus, cobtorin could be a novel inhibitor and an attractive tool for further investigation of the mechanism that enables cortical microtubules to guide the parallel deposition of cellulose microfibrils.