Separation of viable T and B lymphocytes using a cytochemical stain, Hoechst 33342.
Separation of viable T and B lymphocytes using a cytochemical stain, Hoechst 33342.
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使用细胞化学染色剂 Hoechst 33342 分离活的 T 和 B 淋巴细胞。
DOI：
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发表时间：
1980
影响因子：
3.2
通讯作者：
M. Loken
中科院分区：
文献类型：
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作者：
M. Loken
提供的数据表明，组织化学染色Hoeschst 33342可以用来区分小鼠体内存活的B和T淋巴细胞。用Hoechst 33342荧光激活细胞分选仪对不同淋巴组织的细胞染色进行定量分析表明，可以识别两类细胞。在脾中，大约60%的淋巴细胞在1微克/毫升的Hoechst 33342中可以被分类为明亮染色，而在淋巴结中只有40%的细胞被明亮染色。胸腺细胞仅表现出暗淡的染色轮廓。用流式细胞仪将这两个群体从脾中分离出来，并对它们进行细胞表面抗原标记的重新分析，结果显示，Hoechst 33342染色明亮的淋巴细胞主要是免疫球蛋白阳性，而染色较弱的细胞表达Thy1.2。这表明组织化学染色与细胞表面的经典免疫标记物直接相关。
Data are presented that show that a histochemical stain, Hoeschst 33342, can be used to discriminate between viable B and T lymphocytes in the mouse. Quantitative analysis of the staining of cells from various lymphoid tissues with Hoechst 33342 using a Fluorescence-Activated Cell Sorter (FACS) indicates that two populations of cells can be identified. In the spleen approximately 60% of the lymphocytes can be classified as brightly stained with 1 microgram/ml of Hoechst 33342, while in the lymph node only 40% of the cells stain brightly. Thymocytes exhibit only the dull staining profile. Separation of these two populations from the spleen using the FACS and reanalyzing them for cell surface antigenic markers shows that the lymphocytes stained brightly with Hoechst 33342 are predominantly immunoglobulin positive, while the cells that stain less brightly express Thy 1.2. This indicates that a histochemical stain correlates directly with classical immunological markers on cell surfaces.