Cloning of tomato nuclear ribosomal DNA. rDNA organization in leaves and suspension-cultured cells
Cloning of tomato nuclear ribosomal DNA. rDNA organization in leaves and suspension-cultured cells
复制标题
番茄核核糖体 DNA 的克隆。
DOI：
10.1016/0168-9452(89)90167-2
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发表时间：
1989
期刊：
影响因子：
5.2
通讯作者：
Michael Metzlaff
中科院分区：
文献类型：
--
作者：
B. Dobrowolski;K. Glund;Michael Metzlaff
用EcoRI酶切番茄核DNA的rDNA片段与克隆的中性曲霉rDNA进行交叉杂交。分别克隆和鉴定了两个编码18S和25S rRNA的片段。利用它们与特异性裂解核DNA杂交，研究了番茄细胞悬浮培养中rRNA基因的结构组织。发现的重复单位在限制性位点和大小上是可变的，基本单位为8.6 kbp。此外，分析清楚地表明，从番茄叶片（Lycopersicon esculentumcv.）中分离的核DNA。和由此衍生的培养细胞在核糖体DNA的结构组织上有显著的差异。
The rDNA fragments of EcoRI digested tomato nuclear DNA were detected by cross-hybridization with clonedAspergillus nidulansrDNA. Two fragments coding for 18S and 25S rRNA, respectively were cloned and characterized. They were used to study structural organization of rRNA genes of a tomato cell suspension culture by hybridization with specifically cleaved nuclear DNA. The repeating units found were variable in restriction sites and in size with a basic unit of 8.6 kbp. Additionally, the analysis clearly demonstrates that nuclear DNA isolated from tomato leaves (Lycopersicon esculentumcv. Lukullus) and cultured cells derived therefrom bear significant differences in the structural organization of their ribosomal DNA.