Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue
Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue
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DOI：
10.1002/jcb.20904
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发表时间：
2006-12-01
影响因子：
4
通讯作者：
Bunnell, Bruce A.
中科院分区：
文献类型：
--
作者：
Izadpanah, Reza;Trygg, Cynthia;Bunnell, Bruce A.
在这些研究中，评估了从两种不同组织（骨髓和脂肪组织）分离的间充质干细胞（MSCs）的生物学特性。比较了来源于人和非人灵长类动物（恒河猴）组织来源的MSC。结果表明，从恒河猴骨髓中分离的MSCs（rBMSCs）和从人脂肪组织中分离的MSCs（hASCs）比从恒河猴脂肪组织中分离的MSCs（rASCs）和从人骨髓中分离的MSCs（hBMSCs）具有更相似的生物学特性。体外生长动力学分析显示rBMSCs和hASCs的倍增时间较短。rBMSCs和hASCs比其他MSCs经历了更多的群体增殖。所有来源的骨髓间充质干细胞在长期培养中端粒酶活性均显著降低，但仍保持其平均端粒长度。所有MSC均表达胚胎干细胞标志物Oct-4、雷克斯-1和Sox-2，至少传10代。MSC类型的早期群体显示出相似的多谱系分化能力。然而，只有rBMSCs和hASCs在更高的传代次数下保持更高的分化效率。来自这两个物种和组织来源的MSC的总体体外表征揭示了高水平的共同生物学特性。然而，结果也显示出明显的生物学差异。
The biologic characteristics of mesenchymal stem cells (MSCs) isolated from two distinct tissues, bone marrow and adipose tissue were evaluated in these studies. MSCs derived from human and non-human primate (rhesus monkey) tissue sources were compared. The data indicate that MSCs isolated from rhesus bone marrow (rBMSCs) and human adipose tissue (hASCs) had more similar biologic properties than MSCs of rhesus adiposetissue (rASCs) and human bone marrow MSCs (hBMSCs). Analyses of in vitro growth kinetics revealed shorter doubling time for rBMSCs and hASCs. rBMSCs and hASCs underwent significantly more population doublings than the other MSCs. MSCs from all sources showed a marked decrease in telomerase activity over extended culture; however, they maintained their mean telomere length. All of the MSCs expressed embryonic stem cell markers, Oct-4, Rex-1, and Sox-2 for at least 10 passages. Early populations of MSCs types showed similar multilineage differentiation capability. However, only the rBMSCs and hASCs retain greater differentiation efficiency at higher passages. Overall in vitro characterization of MSCs from these two species and tissue sources revealed a high level of common biologic properties. However, the results demonstrate clear biologic distinctions, as well.