Cloning and expression pattern of the lysozyme C gene in zebrafish
Cloning and expression pattern of the lysozyme C gene in zebrafish
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DOI：
10.1016/s0925-4773(01)00658-x
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发表时间：
2002-04-01
影响因子：
2.6
通讯作者：
Wen, ZL
中科院分区：
文献类型：
--
作者：
Liu, F;Wen, ZL
在此，我们报道斑马鱼溶菌酶C基因的分离及其发育表达模式。氨基酸序列分析表明，斑马鱼溶菌酶C蛋白与小鼠、人类、鸡和鲤鱼的对应蛋白具有约37 - 80%的相似性。整胚原位杂交显示溶菌酶C基因在巨噬细胞中表达，因为其表达与已知的髓系标记物L - plastin和PU.1共定位。在受精后20小时（hpf），大多数溶菌酶C阳性细胞位于卵黄囊和头部间充质，而不在中间细胞团，这支持了原始巨噬细胞起源于卵黄囊的观点（《发育》126卷（1999年）3735页）。在受精后36小时，溶菌酶C阳性细胞散布在头部和卵黄囊内，并开始出现在轴静脉的尾部。到受精后6天（dpf），溶菌酶C阳性细胞在肾脏中聚集，据报道造血作用在受精后4天在肾脏中发生（《发育动力学》214卷（1999年）323页）。综上所述，我们的结果表明溶菌酶C基因在髓系中特异性表达，这表明它可作为斑马鱼原始和确定性髓系发育遗传筛选的优良标记。（C）2002年爱尔兰爱思唯尔科学有限公司。保留所有权利。
Here, we report isolation and developmental expression pattern of the zebrafish lysozyme C gene. Amino acid sequence analysis showed that the zebrafish lysozyme C protein shared similar to37-80% identities with the mouse, human, chicken, and carp counterparts. Whole-mount in situ hybridization showed that the lysozyme C gene was expressed in macrophages, as its expression was co-localized with the known myeloid lineage markers L-plastin and PU.1. At 20 hours postfertilization (hpf), most of the lysozyme C positive cells were localized in the yolksac and head mesenchyme but not in the intermediate cell mass, supporting the notion that the primitive macrophage originated from the yolksac (Development 126 (1999) 3735). At 36 hpf, the lysozyme C positive cells scattered within the head and yolksac, and began to appear in the caudal part of axial vein. By 6 days postfertilization (dpf), the lysozyme C positive cells accumulated in the kidney where hematopoiesis had been indicated to take place after 4 dpf (Dev. Dyn. 214 (1999) 323). Taken together, our results demonstrate that the lysozyme C gene is specifically expressed in myeloid lineage, suggesting that it could serve as an excellent marker for genetic screening of both primitive and definitive myeloid lineage development in zebrafish. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.