Tissue flow cytometry immunophenotyping in the diagnosis and classification of non-Hodgkin's lymphomas: A retrospective evaluation of 1,792 cases
Tissue flow cytometry immunophenotyping in the diagnosis and classification of non-Hodgkin's lymphomas: A retrospective evaluation of 1,792 cases
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DOI：
10.1002/cyto.b.21065
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发表时间：
2013-03-01
影响因子：
3.4
通讯作者：
Novero, Domenico
中科院分区：
文献类型：
--
作者：
Demurtas, Anna;Stacchini, Alessandra;Novero, Domenico
回顾分析了12年间提交的1792例提示淋巴瘤的实体组织，并将流式细胞术(FC)的结果与组织学结果进行了比较。本报告中记录的病例的最终组织学诊断如下：1270例非霍奇金淋巴瘤(NHL)；17例混合性淋巴瘤；4例NHL合并癌；5例移植后淋巴组织增生性疾病；105例霍奇金淋巴瘤(HL)；8例急性白血病；42例组织癌；以及341种非肿瘤性疾病。除HL外，恶性血液病(1,268/1,304，97.2%)的形态与FC数据有很强的相关性。在B-NHL中，对克隆性限制性B细胞的Fc检测可以识别组织学上不清楚的淋巴瘤，并可以鉴别滤泡性淋巴瘤和反应性增生。FC法与免疫组化法的增殖结果具有较高的相关性(r=0.83P<；0.0001)。在T-NHL中，在肿瘤细胞少于20%的病例中，Fc检测到异常表型可直接进行组织学诊断。在9个病例中，FC建议有必要评估肿瘤人群，但从形态上看并不明显。结果表明，对疑似淋巴瘤的组织标本常规进行Fc检查是诊断NHL的基本辅助手段，可提高组织学评价的性能，从而实现最终诊断。据我们所知，这是FC研究的一系列广泛组织的文献中的第一份报告。(C)2013国际临床细胞计数学会
A retrospective analysis of 1,792 solid tissues suggestive of lymphoma, submitted over a 12-year period, was carried out and flow cytometry (FC) results were compared with histologic findings. The final histologic diagnosis of cases documented in this report is as follows: 1,270 non-Hodgkin's lymphomas (NHL); 17 composite lymphomas; four NHL plus carcinomas; five post-transplant lymphoproliferative disorders; 105 Hodgkin's lymphomas (HL); eight acute leukemias; 42 tissue cancers; and 341 non-neoplastic diseases. A strong correlation between morphology and FC data was observed among hematological malignancies (1,268/1,304, 97.2%) with the exception of HL. Among B-NHL, FC detection of clonally restricted B-cell allowed the identification of lymphomas that were not histologically clear and the differential diagnosis between follicular lymphoma and reactive hyperplasia. A high correlation level (r = 0.83; P < 0.0001) was obtained in comparing proliferation results obtained by FC and immunohistochemistry. Among T-NHL, FC detection of an aberrant phenotype direct histologic diagnosis in cases having less than 20% of neoplastic cells. In nine cases, FC suggested the need to evaluate a neoplastic population, not morphologically evident. Results show that FC routinely performed on tissue samples suspected of lymphomas is a fundamental adjunct to morphology in the diagnosis of NHL and may enhance the performance of the histologic evaluation so as to achieve the final diagnosis. To the best of our knowledge, this is the first report in the literature of a wide series of tissues also studied by FC. (c) 2013 International Clinical Cytometry Society