Reliability of follicle-stimulating hormone measurements in serum.
Reliability of follicle-stimulating hormone measurements in serum.
复制标题
DOI：
10.1186/1477-7827-1-49
复制
发表时间：
2003-06-18
期刊：
影响因子：
--
通讯作者：
Toniolo, Paolo
中科院分区：
文献类型：
--
作者：
Arslan, Alan A;Zeleniuch-Jacquotte, Anne;Toniolo, Paolo
背景：促卵泡激素（FSH）是促性腺激素家族的一员，对卵泡成熟和卵巢类固醇生成至关重要。已知血清FSH水平在绝经前妇女月经周期的不同阶段波动，并在绝经后由于卵巢功能停止而大幅增加。很少有现有的证据来指导研究人员估计血清FSH测量的可靠性。本研究的目的是评估使用正在进行的前瞻性队列——纽约大学妇女健康研究中储存的血清测量FSH的可靠性。方法：60名健康妇女（16名绝经前妇女，44名绝经后妇女），每隔大约1年捐献至少2份血液样本。采用夹心单克隆抗体技术的免疫放射测定法测定血清中卵泡刺激素水平。结果：单对数转换FSH测量的可靠性，由类内相关系数确定，绝经后妇女为0.70(95%可信区间（CI）， 0.55-0.82)，绝经前妇女为0.09 （95% CI, 0-0.54）。结论：这些结果表明，单次测量足以表征绝经后妇女的血清FSH水平，并可能成为流行病学研究的有用工具。然而，对于绝经前妇女，信度系数很低，这表明单次测定不足以可靠地估计妇女的真实平均血清FSH水平，需要重复测量。
BACKGROUND: Follicle-stimulating hormone (FSH), a member of gonadotropin family, is critical for follicular maturation and ovarian steroidogenesis. Serum FSH levels are known to fluctuate during different phases of menstrual cycle in premenopausal women, and increase considerably after the menopause as a result of ovarian function cessation. There is little existing evidence to guide researchers in estimating the reliability of serum FSH measurements. The objective of this study was to assess the reliability of FSH measurement using stored sera from an ongoing prospective cohort--the NYU Women's Health Study.METHODS: Sixty healthy women (16 premenopausal, 44 postmenopausal), who donated at least two blood samples at approximately 1-year intervals were studied. An immunoradiometric assay using a sandwich monoclonal antibodies technique was used to measure FSH levels in serum.RESULTS: The reliability of a single log-transformed FSH measurement, as determined by the intraclass correlation coefficient, was 0.70 for postmenopausal women (95% confidence interval (CI), 0.55-0.82) and 0.09 for premenopausal women (95% CI, 0-0.54).CONCLUSIONS: These results suggest that a single measurement is sufficient to characterize the serum FSH level in postmenopausal women and could be a useful tool in epidemiological research. For premenopausal women, however, the reliability coefficient was low, suggesting that a single determination is insufficient to reliably estimate a woman's true average serum FSH level and repeated measurements are desirable.