Antioxidative ability of lactic acid bacteria
Antioxidative ability of lactic acid bacteria
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DOI：
10.1021/jf981149l
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发表时间：
1999-04-01
影响因子：
6.1
通讯作者：
Yen, CL
中科院分区：
文献类型：
--
作者：
Lin, MY;Yen, CL
研究了19株乳酸菌的抗氧化活性。这些包括嗜酸乳杆菌B、E、N1、4356、LA-1和Farr;保加利亚乳杆菌12 278、448、449、L B、1006和11 842;嗜热链球菌821、MC、573、3641和19 987;以及长双歧杆菌B6和15 708。所有菌株的胞内无细胞提取物表现出抗氧化活性，抗坏血酸自氧化抑制率在7- 12%的范围内。研究了乳酸菌胞内无细胞提取物的金属离子螯合能力、清除活性氧、酶抑制和还原活性等抗氧化机制。S.嗜热菌821对Fe ~（2+）和B的螯合能力最强。在19个供试菌株中，长柄孢霉15708对Cu ~（2+）的螯合能力最强。所有菌株均表现出活性氧清除能力。L.嗜酸乳杆菌E对羟自由基的清除能力最强，而B的清除能力最强，B.龙眼B6的过氧化氢清除能力最强。所有菌株均具有还原活性，大多数菌株具有较好的还原活性。B。在供试的19个菌株中，长柄霉B6的还原活性最高。在酶抑制方面，19株菌均未检测到超氧化物歧化酶活性，金属离子Mn ~（2+）、Fe ~（2+）、Cu ~（2+）、Zn ~（2+）对超氧化物歧化酶活性无诱导作用。
Nineteen strains of lactic acid bacteria were investigated for antioxidative activity. These included Lactobacillus acidophilus B, E, N1, 4356, LA-1, and Farr; Lactobacillus bulgaricus 12 278, 448, 449, Lb, 1006, and 11 842; Streptococcus thermophilus 821, MC, 573, 3641, and 19 987; and Bifidobacterium longum B6 and 15 708. Intracellular cell-free extract of all strains demonstrated antioxidative activity with inhibition rates of ascorbate autoxidation in the range of 7-12%. Antioxidative mechanisms including metal ion chelating ability, scavenge of reactive oxygen species, enzyme inhibition, and reducing activity of intracellular cell-free extract of lactic acid bacteria were studied. S. thermophilus 821 had the highest metal ion chelating ability for Fe2+, and B. longum 15 708 showed the highest Cu2+ chelating ability among the 19 strains-tested. All strains demonstrated reactive oxygen species scavenging ability. L. acidophilus E showed, the highest hydroxyl radical scavenging ability, and B. longum B6 had the best hydrogen peroxide scavenging ability. Reducing activity was also found in all strains, Most of the strains tested demonstrated excellent reducing activity. B. longum B6 showed the highest reducing activity among the 19 strains tested. In enzyme inhibition, superoxide dismutase activity was not found in these 19 strains, and the activity of superoxide dismutase was not induced when metal ion Mn2+, Fe2+, or Cu2+Zn2+ was present.