Unfertilized Xenopus eggs die by Bad-dependent apoptosis under the control of Cdk1 and JNK.
Unfertilized Xenopus eggs die by Bad-dependent apoptosis under the control of Cdk1 and JNK.
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DOI：
10.1371/journal.pone.0023672
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Jessus C
中科院分区：
文献类型：
--
作者：
Du Pasquier D;Dupré A;Jessus C
排卵的卵子具有在有限时间内被抑制的母体凋亡执行机制。受精卵关闭了这个定时炸弹，而老化的未受精卵和孤雌激活的卵无法停止计时器并死亡。为了研究触发卵自杀决定的分子钟的性质，我们在这里介绍了爪蟾卵作为研究未受精卵死亡的体内系统。我们报告说，排卵后，一些卵子留在女性体内，它们通过细胞凋亡死亡。同样，在外部培养基中回收的未受精卵在72小时内死亡。我们发现，死亡过程取决于细胞色素c的释放和caspase的激活。细胞凋亡机制在受精前的减数分裂成熟过程中启动。死亡途径不依赖于ERK，但依赖于通过激酶Cdk1和JNK的控制激活Bad磷酸化。总之，一个未受精的爪蟾卵的默认命运是死亡的线粒体依赖细胞凋亡激活减数分裂成熟过程中。
Ovulated eggs possess maternal apoptotic execution machinery that is inhibited for a limited time. The fertilized eggs switch off this time bomb whereas aged unfertilized eggs and parthenogenetically activated eggs fail to stop the timer and die. To investigate the nature of the molecular clock that triggers the egg decision of committing suicide, we introduce here Xenopus eggs as an in vivo system for studying the death of unfertilized eggs. We report that after ovulation, a number of eggs remains in the female body where they die by apoptosis. Similarly, ovulated unfertilized eggs recovered in the external medium die within 72 h. We showed that the death process depends on both cytochrome c release and caspase activation. The apoptotic machinery is turned on during meiotic maturation, before fertilization. The death pathway is independent of ERK but relies on activating Bad phosphorylation through the control of both kinases Cdk1 and JNK. In conclusion, the default fate of an unfertilized Xenopus egg is to die by a mitochondrial dependent apoptosis activated during meiotic maturation.
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DOI：
10.1073/pnas.251194298
发表时间：
2001-11-20
影响因子：
11.1
作者：
Bennett, BL;Sasaki, DT;Anderson, DW
通讯作者：
Anderson, DW
影响因子：
4.8
作者：
De Smedt, V;Poulhe, R;Ozon, R
通讯作者：
Ozon, R
影响因子：
9.2
作者：
Gross, SD;Schwab, MS;Maller, JL
通讯作者：
Maller, JL
DOI：
10.1073/pnas.77.1.462
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HARA, K;TYDEMAN, P;KIRSCHNER, M
通讯作者：
KIRSCHNER, M
影响因子：
11.4
作者：
Dupré, A;Jessus, C;Haccard, O
通讯作者：
Haccard, O