A COMPLEX FORMED BETWEEN CELL COMPONENTS AND AN HSV STRUCTURAL POLYPEPTIDE BINDS TO A VIRAL IMMEDIATE EARLY GENE REGULATORY DNA-SEQUENCE
A COMPLEX FORMED BETWEEN CELL COMPONENTS AND AN HSV STRUCTURAL POLYPEPTIDE BINDS TO A VIRAL IMMEDIATE EARLY GENE REGULATORY DNA-SEQUENCE
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DOI：
10.1016/s0092-8674(88)80035-7
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发表时间：
1988-02-12
期刊：
影响因子：
64.5
通讯作者：
CAMPBELL, MEM
中科院分区：
文献类型：
--
作者：
PRESTON, CM;FRAME, MC;CAMPBELL, MEM
Transcription of HSV immediate early genes is stimulated by a virus structural protein, Vmw65, in a process that requires specific recognition of the sequence TAATGARAT (R = purine). Upon incubation of nuclear extracts of HSV-infected cells with a short DNA fragment containing TAATGARAT, a novel virus-induced protein-DNA complex (named IEC) was detected. Addition of virion extract, containing Vmw65, to nuclear extract from uninfected cells also results in the formation of IEC. Vmw65 is a component of IEC, which contains proteins bound specifically to TAATGARAT. Thus, Vmw65 and cellular factors combine to form a sequence-specific DNA-binding complex. This system provides a model for studies of the regulation of inducible cellular genes.
