Characterization of murine lymphokine-activated killer cell cultures separated according to cell size.
Characterization of murine lymphokine-activated killer cell cultures separated according to cell size.
复制标题
根据细胞大小分离的鼠淋巴因子激活的杀伤细胞培养物的表征。
DOI：
--
复制
发表时间：
1993
影响因子：
4.3
通讯作者：
Richard G. Miller
中科院分区：
文献类型：
--
作者：
B. Chadwick;Gerard Brady;Richard G. Miller
小鼠脾细胞在高浓度的淋巴因子白细胞介素2中孵育导致称为淋巴因子激活的杀伤细胞的细胞毒性效应细胞的发展。在这些培养物中形成两个群体的母细胞，一个是T起源的，一个是NK起源的。T原始细胞为CD 8+，FcR γ III-，并表现出轻微增加的前向角光散射（FSC）和侧向角光散射（SSC）。它们对肿瘤靶标YAC-1具有很少或没有溶瘤活性。NK原始细胞为CD 8-、FcR γ III+，并表现出显著增加的FSC和SSC。它们实际上含有针对肿瘤靶标YAC-1的所有裂解活性。基于沉降速度将培养物分成多个级分，沉降速度主要基于细胞大小分离细胞。沉降分离将T细胞和NK细胞都分解为静息（G 0）小淋巴细胞和循环母细胞。NK母细胞比所有CD 8+细胞沉降得快得多，使得大部分溶瘤活性可以相对不含CD 8+细胞地分离。
Incubation of murine splenocytes in high concentrations of the lymphokine interleukin 2 leads to the development of cytotoxic effector cells termed lymphokine-activated killer cells. Two populations of blast cells develop within these cultures, one of T origin and one of NK origin. The T blasts are CD8+, FcR gamma III- and exhibit slightly increased forward angle light scatter (FSC) and side angle light scatter (SSC). They have little or no oncolytic activity against the tumor target YAC-1. The NK blasts are CD8-, FcR gamma III+ and exhibit markedly increased FSC and SSC. They contain virtually all the lytic activity against the tumor target YAC-1. Cultures were split into multiple fractions on the basis of sedimentation velocity, which separates cells primarily on the basis of cell size. The sedimentation separation resolved both T and NK cells into resting (G0) small lymphocytes and cycling blast cells. NK blasts sedimented much more rapidly than all CD8+ cells such that most of the oncolytic activity could be separated relatively free of CD8+ cells.