Activity, diversity and population size of ammonia-oxidising bacteria in oil-contaminated landfarming soil
Activity, diversity and population size of ammonia-oxidising bacteria in oil-contaminated landfarming soil
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DOI：
10.1016/j.femsle.2005.06.057
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发表时间：
2005-09-01
影响因子：
2.1
通讯作者：
Romantschuk, M
中科院分区：
文献类型：
--
作者：
Kurola, J;Salkinoja-Salonen, M;Romantschuk, M
对1999年和2000年两个生长季节中石油污染的农田土壤中存在的化学营养型氨氧化细菌（AOB）进行了研究。通过定量聚合酶链式反应（实时PCR）和潜在铵氧化速率（0.05-0.28μg NO2--N）测定AOB（4-9 x 10（5））细胞g（-1）干土）的数量和潜在铵氧化率（0.05-0.28μg NO2--N）。 g(-1)的干燥土壤h(-1))表明存在稳定的AOB种群。 PCR 扩增的 AOB 16S rRNA 基因的变性梯度凝胶电泳 (DGGE) 分析和序列分析显示，类亚硝化螺菌序列在簇 2 和簇 3 中占主导地位。目前来自长期受石油污染的农田土壤的结果支持了类亚硝化螺菌 AOB 在陆地环境中的重要性。 (c) 2005 年欧洲微生物学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Chemolithotrophic ammonia-oxidising bacteria (AOB) present in oil-contaminated landfarming soil were studied over two growing seasons in 1999 and 2000. The number of AOB (4-9 x 10(5)) cells g(-1) of dry soil) determined with the quantitative polymerase chain reaction (real-time PCR) and the rate of potential ammonium oxidation (0.05-0.28 mu g NO2--N g(-1) of dry soil h(-1)) indicated the presence of stable AOB populations. Denaturing gradient gel electrophoresis (DGGE) profiling and sequence analysis of PCR-amplified AOB 16S rRNA genes showed dominance of Nitrosospira-like sequences in clusters 2 and 3. The present results from the chronically oil-contaminated landfarming soil support the suggested importance of Nitrosospira-like AOB in terrestrial environments. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.