Biomonitoring of polycyclic aromatic hydrocarbons from coke oven emissions and reproductive toxicity in nonsmoking workers.
Biomonitoring of polycyclic aromatic hydrocarbons from coke oven emissions and reproductive toxicity in nonsmoking workers.
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DOI：
10.1016/j.jhazmat.2012.11.008
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发表时间：
2013-01
影响因子：
13.6
通讯作者：
H. Jeng;C. Pan;Wen-Yi Lin;Ming-Tsang Wu;Steven L. Taylor;G. Chang-Chien;Guodong Zhou;N. Diawara
中科院分区：
文献类型：
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作者：
H. Jeng;C. Pan;Wen-Yi Lin;Ming-Tsang Wu;Steven L. Taylor;G. Chang-Chien;Guodong Zhou;N. Diawara
横断面研究的目的是评估暴露于焦炉排放的多环芳烃（PAHs）是否有助于改变非吸烟工人的精液质量和精子DNA完整性。台湾某钢铁厂不吸烟的焦炉工人作为暴露组（高暴露组为顶炉工人，低暴露组为侧炉工人），工厂管理人员和安全人员作为对照组。进行了暴露评估，以确定多环芳烃的颗粒和气相水平以及尿中1-羟基芘（1-OHP）水平。根据世卫组织指南分析精液质量。测量DNA碎片和大体积DNA加合物以评估精子DNA完整性。暴露组和对照组在精子浓度、活力和DNA片段上没有显著差异。与对照组相比，高暴露组的正常形态百分比显著降低（p=0.0001）。暴露组检测到大量DNA加合物，显著高于对照组（p=0.04）。暴露于焦炉排放的多环芳烃可能会导致精子中体积庞大的DNA加合物水平增加。
The objective of the cross-sectional study was to assess whether exposure to polycyclic aromatic hydrocarbons (PAHs) from coke oven emissions contributed to alteration of semen quality and sperm DNA integrity in nonsmoking workers. Nonsmoking coke oven workers from a steel plant in Taiwan served as the exposure groups (topside-oven workers for the high exposure group and side-oven workers for the low exposure group), and administrators and security personnel in the plant served as the control. An exposure assessment was conducted to determine both particulate and gaseous phase of PAH levels and urinary 1-hydroxypyrene (1-OHP) levels. Semen quality was analyzed according to WHO guidelines. DNA fragmentation and bulky DNA adducts were measured to assess sperm DNA integrity. There was no significant difference in sperm concentrations, vitality, and DNA fragmentation between the exposed group and the control. The high exposure group experienced significantly lower percentages of normal morphology as compared with the control (p=0.0001). Bulky DNA adducts were detected in the exposed group that were significant higher than the control (p=0.04). Exposure to PAHs from coke-oven emissions could contribute to increased levels of bulky DNA adducts in sperm.